Anti-Collagen III antibody [Col-29] (ab82354)

Overview

• Product nameAnti-Collagen III antibody [Col-29]See all Collagen III primary antibodies ...
• Description
  Mouse monoclonal [Col-29] to Collagen III
• Tested applicationsIHC-P, WB, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Human collagen type III

• Positive controlIHC-P: human skin FFPE tissue sections

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
  Constituents: 1.2% Sodium acetate, 2mg BSA
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberCol-29
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
• Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
  Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
  Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02461 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 12825 Mouse
  • Entrez Gene: 84032 Rat
  • Omim: 120180 Human
  • SwissProt: P02461 Human
  • SwissProt: P02461 Human

  • SwissProt: P02461 Human
  • SwissProt: P08121 Mouse
  • SwissProt: P13941 Rat
  • Unigene: 443625 Human
  • Unigene: 249555 Mouse
  • Unigene: 3247 Rat

  see all

• Alternative names
  CO3A1_HUMAN antibodyCOL 3A1 antibodyCOL3A1 antibody

  Collagen alpha 1(III) chain antibodyCollagen alpha-1(III) chain antibodyCollagen III alpha 1 chain precursor antibodyCollagen III alpha 1 polypeptide antibodyCollagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibodyCollagen type III alpha 1 antibodyCollagen type III alpha antibodyEDS4A antibodyEhlers Danlos syndrome type IV, autosomal dominant antibodyFetal collagen antibodyType III collagen antibody

  see all

Anti-Collagen III antibody [Col-29] images

• 

  Western blot - Collagen III antibody [Col-29] (ab82354)
  All lanes : Anti-Collagen III antibody [Col-29] (ab82354)
  
  Lane 1 : HT1080 Whole cell lysate
  Lane 2 : Human Colo320 Whole cell lysate
  
  
  Predicted band size : 138 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [Col-29] (ab82354)
  IHC image of Collagen III staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82354, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.