Anti-Collagen III antibody [Col-29] (ab82354)

Overview

  • Product nameAnti-Collagen III antibody [Col-29]See all Collagen III primary antibodies ...
  • Description
    Mouse monoclonal [Col-29] to Collagen III
  • Tested applicationsIHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Full length protein corresponding to Human Collagen III aa 1-1466.
    Database link: P02461

  • Positive control
    • IHC-P: Human skin FFPE tissue sections WB: Human Colo320 Whole cell lysate, HT1080 Whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 138 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

Target

  • FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Anti-Collagen III antibody [Col-29] images

  • IHC image of Collagen III staining in Human normal skin (ab30166) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82354, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Collagen III antibody [Col-29] (ab82354)

    Lane 1 : HT1080 Whole cell lysate
    Lane 2 : Human Colo320 Whole cell lysate


    Predicted band size : 138 kDa

References for Anti-Collagen III antibody [Col-29] (ab82354)

ab82354 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 60000µg/mL · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Sample Rabbit Tissue sections (Intervertebral Disc (all cells particularly NP))
Specification Intervertebral Disc (all cells particularly NP)
Permeabilization Yes - 1X TBS/0.1% Tween-20
Fixative Formaldehyde
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Verified customer

Submitted May 30 2014

We do not have an order form Lucerna for these antibodies. Plese check with them.

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100%ABR-T71OF für ab23446
100%ABR-T71OS für ab82354

Ablaufdatum: 31. August 2012

Es freut uns zu hören, dass Sie unser Angebot unsere Antikörper ab23446 und ab82354 inRatteund Schwei...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, ...

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I have checked the homology between human and pig for collagen III. It is 93% for the full length protein, therefore there is a high chance of crossreactivity. The following collagen III antibodies were tested in...

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Thank you for your inquiry. The testing laboratory confirmed that the band in WB application is about 70 kDa, because it is run denatured-reduced conditions. If you were to run native, non-reduced conditions, a higher MW would be expected. I hop...

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