Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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ab6310 has been referenced in 13 publications.
Publishing research using ab6310? Please let us know so that we can cite the reference in this datasheet
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ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.
This image is courtesy of an Abreview submitted by Birgitta Weijdegard
All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : Reduced, denaturated Collagen III protein
Lane 2 : Native Collagen III protein
Lysates/proteins at 5 µg per lane.
Secondary
Goat anti-mouse HRP at 1/2000 dilution
developed using the ECL technique
Exposure time : 1 minute
Image courtesy of Michaela Leyh by Abreview.
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