Full length native protein (purified) (Human).
Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab7778 as a replacement.
Our Abpromise guarantee covers the use of ab6310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot | Use at an assay dependent concentration. | |
ELISA | Use at an assay dependent concentration. | |
IHC-Fr | 1/4000. | |
WB | 1/100 - 1/1000. | |
IHC-P | 1/600. | |
ICC/IF | Use at an assay dependent concentration. PubMed: 25136258 | |
Indirect ELISA | Use at an assay dependent concentration. |
Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.
Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"