Anti-Collagen III antibody [FH-7A] (ab6310)

Overview

• Product nameAnti-Collagen III antibody [FH-7A]See all Collagen III primary antibodies ...
• Description
  Mouse monoclonal [FH-7A] to Collagen III
• SpecificityThe antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.
• Tested applicationsDot Blot, ELISA, IHC-Fr, WB, IHC-P, Indirect ELISA more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Full length native protein (purified) (Human).

• Positive control
  • IHC: Human skin sections. WB: recombinant collagen III.
• General notes

  Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• Storage bufferPreservative: 0.097% Sodium azide
  Constituent: Whole serum
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberFH-7A
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

Applications

Target

• FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
• Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
  Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
  Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

• Target information above from: UniProt accession P02461 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 1281 Human
  • Entrez Gene: 84032 Rat
  • Omim: 120180 Human
  • SwissProt: P02461 Human
  • SwissProt: P13941 Rat
  • Unigene: 443625 Human
  • Unigene: 3247 Rat
• Alternative names
  • CO3A1_HUMAN antibody
  • COL 3A1 antibody
  • COL3A1 antibody

  • Collagen alpha 1(III) chain antibody
  • Collagen alpha-1(III) chain antibody
  • Collagen III alpha 1 chain precursor antibody
  • Collagen III alpha 1 polypeptide antibody
  • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
  • Collagen type III alpha 1 antibody
  • Collagen type III alpha antibody
  • EDS4A antibody
  • Ehlers Danlos syndrome type IV, autosomal dominant antibody
  • Fetal collagen antibody
  • Type III collagen antibody

  see all

Anti-Collagen III antibody [FH-7A] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

  Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)This image is courtesy of an Abreview submitted by Birgitta Weijdegard
  ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

  Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

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  Western blot - Collagen III antibody [FH-7A] (ab6310)Image courtesy of Michaela Leyh by Abreview.
  All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
  
  Lane 1 : Reduced, denaturated Collagen III protein
  Lane 2 : Native Collagen III protein
  
  Lysates/proteins at 5 µg per lane.
  
  Secondary
  Goat anti-mouse HRP at 1/2000 dilution
  developed using the ECL technique
  
  Exposure time : 1 minute

  Image courtesy of Michaela Leyh by Abreview.