Overview

  • Product nameAnti-Collagen III antibody [FH-7A]
    See all Collagen III primary antibodies
  • Description
    Mouse monoclonal [FH-7A] to Collagen III
  • SpecificityThe antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.
  • Tested applicationsSuitable for: Dot blot, ELISA, IHC-Fr, WB, IHC-P, ICC/IF, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • IHC: Human skin sections. WB: recombinant collagen III.
  • General notes

    Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

Properties

Applications

Our Abpromise guarantee covers the use of ab6310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/4000.
WB 1/100 - 1/1000.
IHC-P 1/600.
ICC/IF Use at an assay dependent concentration. PubMed: 25136258
Indirect ELISA Use at an assay dependent concentration.

Target

  • FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type III collagen antibody
    • Alpha1 (III) collagen antibody
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha 1 chain antibody
    • Collagen type III alpha antibody
    • Collagen, fetal antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Anti-Collagen III antibody [FH-7A] images

  • Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

  • All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

    Lane 1 : Reduced, denaturated Collagen III protein
    Lane 2 : Native Collagen III protein

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat anti-mouse HRP at 1/2000 dilution
    Developed using the ECL technique

    Exposure time : 1 minute

    Image courtesy of Michaela Leyh by Abreview.

  • ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

    See Abreview

  • Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

References for Anti-Collagen III antibody [FH-7A] (ab6310)

This product has been referenced in:
  • Fang CL  et al. Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin. Int J Mol Sci 17:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27428951) »
  • Li CW  et al. Silver nanoparticles/chitosan oligosaccharide/poly(vinyl alcohol) nanofiber promotes wound healing by activating TGFß1/Smad signaling pathway. Int J Nanomedicine 11:373-86 (2016). WB, IHC-Fr . Read more (PubMed: 26855575) »

See all 33 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (purified protein and cell culture supernatant)
Specification purified protein and cell culture supernatant
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type Sandwich (Capture)
Username

Patricia Carulla

Verified customer

Submitted Jun 28 2017

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (First trimester human decidua)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: 0.1% (W/V) Trypsin
Permeabilization No
Specification First trimester human decidua
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
Username

Ruhul Choudhury

Verified customer

Submitted Feb 13 2017

Thank you for contacting us. We offer either replacement or refund. As you have already received compensation in the form of a replacement antibody, you would be ineligible for a refund. Please note that your replacement product is covered by our Ab...

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Wie besprochen, habe ich eine kostenlose Ersatzlieferung mit ab34710für Sie in Auftrag gegeben. Sie hat die Referenznummer x und sollte in den nächsten Tagen direkt bei Ihnen im Labor ankommen. <...

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Es tut mir leid, dass Sie Probleme mit unserem Collagen III Antikörper hatten. Nachdem ich mir die Details ange...

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