Anti-Collagen III antibody [FH-7A] (ab6310)
- Product nameAnti-Collagen III antibody [FH-7A]See all Collagen III primary antibodies ...
- DescriptionMouse monoclonal [FH-7A] to Collagen III
- SpecificityThe antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.
- Tested applicationsDot Blot, ELISA, IHC-Fr, WB, IHC-P more details
- Species reactivityReacts with: Rat, Human
Full length native protein (purified) (Human).
- Positive controlIHC: Human skin sections. WB: recombinant collagen III.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: Ascites fluid
- Concentration information loading...
- Clonality Monoclonal
- Clone numberFH-7A
- Research Areas
Our Abpromise guarantee covers the use of ab6310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot Blot||Dot: Use at an assay dependent dilution.|
|ELISA||ELISA: Use at an assay dependent dilution.|
|WB||WB: 1/100 - 1/1000.|
- FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
- Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
- Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain.
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- CO3A1_HUMAN antibodyCOL 3A1 antibodyCOL3A1 antibody
- Collagen alpha 1(III) chain antibodyCollagen alpha-1(III) chain antibodyCollagen III alpha 1 chain precursor antibodyCollagen III alpha 1 polypeptide antibodyCollagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibodyCollagen type III alpha 1 antibodyCollagen type III alpha antibodyEDS4A antibodyEhlers Danlos syndrome type IV, autosomal dominant antibodyFetal collagen antibodyType III collagen antibody
Anti-Collagen III antibody [FH-7A] images
ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.
All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : Reduced, denaturated Collagen III protein
Lane 2 : Native Collagen III protein
Lysates/proteins at 5 µg per lane.
Goat anti-mouse HRP at 1/2000 dilution
developed using the ECL technique
Exposure time : 1 minute
Image courtesy of Michaela Leyh by Abreview.
References for Anti-Collagen III antibody [FH-7A] (ab6310)
This product has been referenced in:
- Garcia AG et al. Interferon-? ablation exacerbates myocardial hypertrophy in diastolic heart failure. Am J Physiol Heart Circ Physiol 303:H587-96 (2012). WB ; Mouse . Read more (PubMed: 22730392) »
- Campbell DJ et al. Diastolic dysfunction of aging is independent of myocardial structure but associated with plasma advanced glycation end-product levels. PLoS One 7:e49813 (2012). IHC-Fr ; Human . Read more (PubMed: 23189164) »