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Anti-Collagen III antibody [FH-7A] (ab6310)

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Overview

Product name

Anti-Collagen III antibody [FH-7A]
See all Collagen III products (13) ...

Description

Mouse monoclonal [FH-7A] to Collagen III

Specificity

The antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.

Tested applications

Dot Blot, ELISA, IHC-Fr, WB, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Human

Immunogen

Full length native protein (purified) (Human).

Positive control

IHC: Human skin sections. WB: recombinant collagen III.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites fluid

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

FH-7A

Isotype

IgG1

Research areas

Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310) image (enlarge)

  • Western blot - Collagen III antibody [FH-7A] (ab6310)Western blot - Collagen III antibody [FH-7A] (ab6310) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

Cellular localization

Secreted; extracellular space, extracellular matrix

Alternative names

  • COL 3A1 antibody
  • COL3A1 antibody
  • Collagen alpha 1(III) chain antibody
  • Collagen III alpha 1 chain precursor antibody
  • Collagen III alpha 1 polypeptide antibody
  • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
  • Collagen type III alpha 1 antibody
  • Collagen type III alpha antibody
  • EDS4A antibody
  • Ehlers Danlos syndrome type IV, autosomal dominant antibody
  • Fetal collagen antibody
  • Type III collagen antibody
see all

Database links

Anti-Collagen III antibody [FH-7A] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)

ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

This image is courtesy of an Abreview submitted by Birgitta Weijdegard

See Abreview

  Western blot - Collagen III antibody [FH-7A] (ab6310)

Western blot - Collagen III antibody [FH-7A] (ab6310)

All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

Lane 1 : Reduced, denaturated Collagen III protein
Lane 2 : Native Collagen III protein

Lysates/proteins at 5 µg per lane.

Secondary
Goat anti-mouse HRP at 1/2000 dilution
developed using the ECL technique

Exposure time : 1 minute

Image courtesy of Michaela Leyh by Abreview.

References for Anti-Collagen III antibody [FH-7A] (ab6310)

This product has been referenced in:

  • Chen PSet al. miR-107 promotes tumor progression by targeting the let-7 microRNA in mice and humans. J Clin Invest 121:3442-55 (2011). IHC-P; Human.Read more (PubMed: 21841313) »
  • Dahiya Set al. Elevated levels of active matrix metalloproteinase-9 cause hypertrophy in skeletal muscle of normal and dystrophin-deficient mdx mice. Hum Mol Genet 20:4345-59 (2011).Read more (PubMed: 21846793) »

See all 12 publications for this product

Publishing research using ab6310? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"