|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)](/ps/datasheet/images/ab6310_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Collagen III antibody [FH-7A]
|
| |
See all Collagen III antibodies (14)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [FH-7A] to Collagen III
|
|
Immunogen
|
Full length native protein (purified) (Human).
|
|
Reacts with
(species key)
|
Hu, Rat
|
|
Specificity
|
The antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.
|
|
Tested applications
(see key)
|
Dot, ELISA, IHC-Fr, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Dot: Use at an assay dependant dilution.
ELISA: Use at an assay dependant dilution.
IHC-Fr: 1/4000.
IHC-P: 1/600.
WB: 1/100 - 1/1000. The antibody recognizes a ~ 70 kDa band in reduced denatured preps and a ~ 300 kDa band in non-reduced, non-denatured preps.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
IHC: Human skin sections.
WB: recombinant collagen III.
|
|
Cellular localization
|
Secreted; extracellular space, extracellular matrix
|
|
Research areas
|
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
|
|
Relevance
|
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
|
|
|
Collagen III antibody [FH-7A] (ab6310) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
FH-7A
|
|
Isotype
|
IgG1
|
|
Purity
|
Ascites
|
|
Storage buffer
|
Preservative: 15mM Sodium Azide
Constituents: Ascites fluid Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 2.40 - 3.20 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Collagen III antibody [FH-7A] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Collagen III antibody [FH-7A] images, references, products related to ab6310 and other tools.
|
| |
Collagen III antibody [FH-7A] images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)
ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.
This image is courtesy of an Abreview submitted by Birgitta Weijdegard
See Abreview
|
| |
References for Collagen III antibody [FH-7A] (ab6310)
|
|
This product has been used in: (two most recent references)
Toumpoulis IK et al. Differential expression of collagen type V and XI alpha-1 in human ascending thoracic aortic aneurysms. Ann Thorac Surg 88:506-13 (2009). PubMed: 19632402
Toumpoulis IK et al. Differential expression of collagen type V and XI alpha-1 in human ascending thoracic aortic aneurysms. Ann Thorac Surg 88:506-13 (2009). WB; Human. PubMed: 19632402
See all 10 publication references for this product.
If you publish research using ab6310 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Collagen III
|
| |
Collagen III antibody [FH-7A] - more information
|
Customer reviews (feedback) regarding Collagen III antibody [FH-7A] |
Customer FAQs regarding Collagen III antibody [FH-7A] |
Protocols for Collagen III antibody [FH-7A] |
Price and availability of products related to Collagen III antibody [FH-7A] |
| |
Collagen III antibody [FH-7A] - related products:
|
|
|
| |
Collagen III antibody [FH-7A] - other tools:
|
Contact Abcam with a Technical Enquiry about Collagen III antibody [FH-7A] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)](/index.html?datasheet=6310#ab6310_1.jpg)
