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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)
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Product Name 

Collagen III antibody [FH-7A]

 

See all Collagen III antibodies (14)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [FH-7A] to Collagen III

Immunogen 

Full length native protein (purified) (Human).

Reacts with 
(species key)

Hu, Rat

Specificity 

The antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.

Tested applications 
(see key)

Dot, ELISA, IHC-Fr, IHC-P, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ELISA

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IHC-P

2 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


IHC-P
(read Abreview)


Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependant dilution.
ELISA: Use at an assay dependant dilution.
IHC-Fr: 1/4000.
IHC-P: 1/600.
WB: 1/100 - 1/1000. The antibody recognizes a ~ 70 kDa band in reduced denatured preps and a ~ 300 kDa band in non-reduced, non-denatured preps.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IHC: Human skin sections.
WB: recombinant collagen III.

Cellular localization 

Secreted; extracellular space, extracellular matrix

Research areas 

Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen

Relevance 

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

 

Collagen III antibody [FH-7A] (ab6310)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1281    (Human)
84032    (Rat)

Omim

    

120180    (Human)

SwissProt

    

P02461    (Human)
P13941    (Rat)

Unigene

    

443625    (Human)
3247    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

FH-7A

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Ascites fluid

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 2.40 - 3.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Collagen III antibody [FH-7A] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Collagen III antibody [FH-7A] images, references, products related to ab6310 and other tools.

 

Collagen III antibody [FH-7A] images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)

ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

This image is courtesy of an Abreview submitted by Birgitta Weijdegard

See Abreview

 

References for Collagen III antibody [FH-7A] (ab6310)

Specific publication references   This product has been used in: (two most recent references)

Toumpoulis IK et al. Differential expression of collagen type V and XI alpha-1 in human ascending thoracic aortic aneurysms. Ann Thorac Surg 88:506-13 (2009). PubMed: 19632402

Toumpoulis IK et al. Differential expression of collagen type V and XI alpha-1 in human ascending thoracic aortic aneurysms. Ann Thorac Surg 88:506-13 (2009). WB; Human. PubMed: 19632402


See all 10 publication references for this product.


If you publish research using ab6310 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Collagen III

 

Collagen III antibody [FH-7A] - more information

Customer reviews (feedback) regarding Collagen III antibody [FH-7A]

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Price and availability of products related to Collagen III antibody [FH-7A]

 

Collagen III antibody [FH-7A] - related products:

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Other Collagen III Antibodies

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Collagen III antibody [FH-7A] - other tools:

Contact Abcam with a Technical Enquiry about Collagen III antibody [FH-7A]

 

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