Anti-Collagen III antibody [FH-7A] (ab6310)
- Datasheet
- Specific References (60)
- Protocols
Overview
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Product nameAnti-Collagen III antibody [FH-7A]
See all Collagen III primary antibodies -
DescriptionMouse monoclonal [FH-7A] to Collagen III
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Host speciesMouse
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Tested applicationsSuitable for: Dot blot, ELISA, IHC-Fr, WB, IHC-P, ICC/IF, Indirect ELISAmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Full length native protein (purified) (Human).
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Positive control
- IHC: Human skin sections. WB: recombinant collagen III.
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General notes
Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab7778 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.097% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
PurityAscites
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ClonalityMonoclonal
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Clone numberFH-7A
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab6310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | 1/4000. | |
| WB | 1/100 - 1/1000. | |
| IHC-P | 1/600. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 25136258 | |
| Indirect ELISA | Use at an assay dependent concentration. |
Target
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FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
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Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. -
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 1281 Human
- Entrez Gene: 84032 Rat
- Omim: 120180 Human
- SwissProt: P02461 Human
- SwissProt: P13941 Rat
- Unigene: 443625 Human
- Unigene: 3247 Rat
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Alternative names
- Alpha 1 type III collagen antibody
- Alpha1 (III) collagen antibody
- CO3A1_HUMAN antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)](http://a.abcam.com/ps/products/6/ab6310/Images/ab6310-219364-ab6310.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.
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![Western blot - Anti-Collagen III antibody [FH-7A] (ab6310)](http://a.abcam.com/ps/products/6/ab6310/Images/Collagen-III-Primary-antibodies-ab6310-3.jpg)
Western blot - Anti-Collagen III antibody [FH-7A] (ab6310)Image courtesy of Michaela Leyh by Abreview.All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : Reduced, denaturated Collagen III protein
Lane 2 : Native Collagen III protein
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat anti-mouse HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 1 minute -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)](http://a.abcam.com/ps/products/6/ab6310/Images/ab6310_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody [FH-7A] (ab6310)This image is courtesy of an Abreview submitted by Birgitta Weijdegardab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells. -
![Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)](http://a.abcam.com/ps/products/6/ab6310/Images/ab6310-219363-ab6310.jpg)
Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.
Protocols
References
This product has been referenced in:
- Ide J & Tokunaga T Rotator cuff tendon-to-bone healing at 12 months after patch grafting of acellular dermal matrix in an animal model. J Orthop Sci 23:207-212 (2018). IHC ; Rat . Read more (PubMed: 29277362) »
- Wu X et al. Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis. Int J Mol Med 41:1992-2002 (2018). Read more (PubMed: 29393361) »
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