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Overview

  • Product nameAnti-Collagen III antibody [FH-7A]See all Collagen III primary antibodies ...
  • Description
    Mouse monoclonal [FH-7A] to Collagen III
  • SpecificityThe antibody specifically recognizes native and denatured recombinant collagen type III. It does not recognise collagen types I, II, IV, V, VI and X and has not been tested against endogenous collagen III.
  • Tested applicationsDot Blot, ELISA, IHC-Fr, WB, IHC-P more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive controlIHC: Human skin sections. WB: recombinant collagen III.

Properties

Applications

Our Abpromise guarantee covers the use of ab6310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot Dot: Use at an assay dependent dilution.
ELISA ELISA: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: 1/4000.
WB WB: 1/100 - 1/1000.
IHC-P IHC-P: 1/600.

Target

  • FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02461 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
      CO3A1_HUMAN antibodyCOL 3A1 antibodyCOL3A1 antibody
      Collagen alpha 1(III) chain antibodyCollagen alpha-1(III) chain antibodyCollagen III alpha 1 chain precursor antibodyCollagen III alpha 1 polypeptide antibodyCollagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibodyCollagen type III alpha 1 antibodyCollagen type III alpha antibodyEDS4A antibodyEhlers Danlos syndrome type IV, autosomal dominant antibodyFetal collagen antibodyType III collagen antibody
    see all

Anti-Collagen III antibody [FH-7A] images

  • ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

    See Abreview

  • All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

    Lane 1 : Reduced, denaturated Collagen III protein
    Lane 2 : Native Collagen III protein

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat anti-mouse HRP at 1/2000 dilution
    developed using the ECL technique

    Exposure time : 1 minute

    Image courtesy of Michaela Leyh by Abreview.

References for Anti-Collagen III antibody [FH-7A] (ab6310)

This product has been referenced in:
  • Garcia AG  et al. Interferon-? ablation exacerbates myocardial hypertrophy in diastolic heart failure. Am J Physiol Heart Circ Physiol 303:H587-96 (2012). WB ; Mouse . Read more (PubMed: 22730392) »
  • Campbell DJ  et al. Diastolic dysfunction of aging is independent of myocardial structure but associated with plasma advanced glycation end-product levels. PLoS One 7:e49813 (2012). IHC-Fr ; Human . Read more (PubMed: 23189164) »

See all 15 Publications for this product

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Thank you for contacting us. We offer either replacement or refund. As you have already received compensation in the form of a replacement antibody, you would be ineligible for a refund. Please note that your replacement product is covered by our Abpro...

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I hope the suggestions may proove useful with ab6310. It may be worth trying citrate antigen retreival with the PFA ...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein und Schafund ab6310 nicht in Schaf getestet.

Falls Sie dies selbst testen möchten, kan...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team an...

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Vielen Dank für Ihre Nachricht.

Wie besprochen, habe ich eine kostenlose Ersatzlieferung mit ab34710für Sie in Auftrag gegeben. Sie hat die Referenznummer x und sollte in den nächsten Tagen direkt bei Ihnen im Labor ankommen.
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Vielen Dank fürIhreNachricht und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unserem Collagen III Antikörper hatten. Nachdem ich mir die Details angeseh...

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Vielen Dank für Ihre Anfrage.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Collagen IIIAntikörper haben.

Wenn der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben (und das scheint offen...

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Thank you for contacting us. We are working on correcting the error for ab6310, as pig has not yet been tested. The immunogen for this product was human collagen III which ihas 93% sequence homology with the pig protein. We do not have any other collagen ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"