Overview

  • Product nameAnti-Collagen III antibody
    See all Collagen III primary antibodies
  • Description
    Rabbit polyclonal to Collagen III
  • SpecificityRat collagen type III 100%, rat collagen I <0.1%; rat collagen type V <10%; rat elastin <0.1% (solid phase RIA at 1:50 dilution).
  • Tested applicationsIHC-P, ICC/IF, ELISA, RIAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length native protein (purified from skin) (Rat).

  • Positive control
    • Rat skin or liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab23746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/600. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.

Target

  • FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

References for Anti-Collagen III antibody (ab23746)

This product has been referenced in:
  • Whaley-Connell AT  et al. Salt loading exacerbates diastolic dysfunction and cardiac remodeling in young female Ren2 rats. Metabolism 62:1761-71 (2013). Rat . Read more (PubMed: 24075738) »
  • Carpe N  et al. Genetic influences on asthma susceptibility in the developing lung. Am J Respir Cell Mol Biol 43:720-30 (2010). Read more (PubMed: 20118217) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I would recommend that you aliquot your antibodies and only dilute when you are ready to use the antibody. Also, I would recommend that you dilute them into PBS, not TBS. Unfortunately, we do not have a protocol specifically...

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Thank you for your enquiry. First, let me direct you a very informative website about IHC called IHC World. The web address is www.ihcworld.com. You can search that website for streptavidin, and then click on the Immunohistochemistry Introduction link....

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I am in touch with the supplier of ab13966 and will let you know any information I receive as soon as possible. Regarding ab23746, the customer can purchase Hyaluronidase from the company Biologo (http://www.biologo.de/englisch/), reference HYA02-50.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"