Overview

  • Product nameAnti-Collagen III antibodySee all Collagen III primary antibodies ...
  • Description
    Rabbit polyclonal to Collagen III
  • Tested applicationsDot Blot, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Full length native protein (purified) (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab24119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot
ELISA
IHC-Fr
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    IHC-Fr: 1/10 if FITC conjugated secondary antibody is used, or 1/20 for secondary-peroxidase conjugates.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
    • Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
      Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
      Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
    • Sequence similaritiesBelongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • Post-translational
      modifications
      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CO3A1_HUMAN antibody
      • COL 3A1 antibody
      • COL3A1 antibody
      • Collagen alpha 1(III) chain antibody
      • Collagen alpha-1(III) chain antibody
      • Collagen III alpha 1 chain precursor antibody
      • Collagen III alpha 1 polypeptide antibody
      • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
      • Collagen type III alpha 1 antibody
      • Collagen type III alpha antibody
      • EDS4A antibody
      • Ehlers Danlos syndrome type IV, autosomal dominant antibody
      • Fetal collagen antibody
      • Type III collagen antibody
      see all

    Anti-Collagen III antibody images

    • ab24119 staining Collagen III in pig epithelial tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone and then incubated with ab24119 at a 1/10 dilution for 1 hour at 25°C. The secondary used was ab98650 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain, used at a 1/1000 dilution. DAPI (Blue) stained for nuclei. Far Red stains for Collagen III.

      See Abreview

    References for Anti-Collagen III antibody (ab24119)

    This product has been referenced in:
    • Müller NF  et al. Trimeric autotransporter adhesin-dependent adherence of Bartonella henselae, Bartonella quintana, and Yersinia enterocolitica to matrix components and endothelial cells under static and dynamic flow conditions. Infect Immun 79:2544-53 (2011). IF . Read more (PubMed: 21536788) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    I have checked the homology between human and pig for collagen III. It is 93% for the full length protein, therefore there is a high chance of crossreactivity. The following collagen III antibodies were tested in...

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    Thank you for your message. I am pleased to help answer your questions.

    1. This antibody is a polyclonal antibody raised against the full length human protein. Therefore, as a polyclonal antibody it will be detecting epitopes all along the l...

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    Thank you for contacting us. We are working on correcting the error for ab6310, as pig has not yet been tested. The immunogen for this product was human collagen III which ihas 93% sequence homology with the pig protein. We do not have any other collag...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Pig Tissue sections (Epithelial)
    Specification Epithelial
    Fixative Acetone
    Permeabilization No
    Username

    Mr. Daniel Holod-Betz

    Verified customer

    Submitted Mar 09 2012

    DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab24119 in IHC-Fr on Swine. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"