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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Anti-Collagen III antibody
See all Collagen III products (13) ...
Description
Rabbit polyclonal to Collagen III
Specificity
Detects endogenous levels of total collagen III protein.
Tested applications
ICC/IF, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) from an internal region.
Positive control
Human pancreas tissue and HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
ELISA
IHC-P
Application notes
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol, using 10 MM citrate buffer, pH 6.0.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Cellular localization
Secreted; extracellular space, extracellular matrix
Alternative names
- COL 3A1 antibody
- COL3A1 antibody
- Collagen alpha 1(III) chain antibody
see all
Database links
- Entrez Gene: 1281 Human
- Entrez Gene: 12825 Mouse
- Entrez Gene: 84032 Rat
- Omim: 120180 Human
- SwissProt: P02461 Human
- SwissProt: P08121 Mouse
- SwissProt: Q7TT32 Mouse
- SwissProt: P13941 Rat
see all
Anti-Collagen III antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Collagen III antibody (ab59436)
Immunohistochemical analysis of Collagen III in human pancreas tissue (paraffin section) using ab59436 (1/500 dilution); in the presence or absence of immuninzing peptide.
Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab59436)
Immunofluorescence analysis of Collagen III in HeLa cells using ab59436; in the presence or absence of immuninzing peptide.
References for Anti-Collagen III antibody (ab59436)
ab59436 has not yet been referenced specifically in any publications.
Publishing research using ab59436? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"