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Immunohistochemistry (Paraffin-embedded sections) - Collagen III antibody (ab59436)
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Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab59436)
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Product Name
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Collagen III antibody
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See all Collagen III antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Collagen III
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Immunogen
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Synthetic peptide (Human) from an internal region.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of total collagen III protein.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P
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Abreviews
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Application notes
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Recommended dilutions
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol, using 10 MM citrate buffer, pH 6.0.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Human pancreas tissue and HeLa cells.
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Cellular localization
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Secreted; extracellular space, extracellular matrix
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Research areas
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Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Relevance
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Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Collagen III antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Collagen III antibody images, references, products related to ab59436 and other tools.
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Collagen III antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Collagen III antibody (ab59436)
Immunohistochemical analysis of Collagen III in human pancreas tissue (paraffin section) using ab59436 (1/500 dilution); in the presence or absence of immuninzing peptide.
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Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab59436)
Immunofluorescence analysis of Collagen III in HeLa cells using ab59436; in the presence or absence of immuninzing peptide.
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Search PubMed (MEDLINE) for references to Collagen III
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Collagen III antibody - more information
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Customer reviews (feedback) regarding Collagen III antibody |
Customer FAQs regarding Collagen III antibody |
Protocols for Collagen III antibody |
Price and availability of products related to Collagen III antibody |
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Collagen III antibody - related products:
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Collagen III antibody - other tools:
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Contact Abcam with a Technical Enquiry about Collagen III antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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