Anti-Collagen III antibody (ab7778)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody (ab7778)

Ab53088 staining Human skin. Staining is localised to the extracellular matrix.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab7778)

ab7778 staining Collagen III in Human saphenous vein-derived myofibroblasts by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton-X100. Prior to staining, cells were washed and blocked in a 2% bovine serum albumin, 0.1% saponin (w=v) solution in PBS, and subsequently incubated for 1 hour with the primary antibody in PBS. Nuclei are stained with Hoechst.

Image courtesy of Riem Vis PW et al, Tissue Eng Part A. 2010 Apr;16(4):1317-27, Fig 4.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.

Image courtesy of Rudolf Jung by Abreview.