Anti-Collagen III antibody (ab7778)

ab7778und Proben vom Schwein

ANSWER:

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab7778 abdecken, und gegen eine erneute Bestellung von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab7778 inSchwein testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in Schwein.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
http://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab7778 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab7778 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
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Die Datenblätter für die zwei passenden sekundären Antikörper, die wir am Telefon besprochen haben,können über diese Links eingesehen werden:

http://www.abcam.com/index.html?datasheet=96983 (or use the following: http://www.abcam.com/index.html?datasheet=96983).

http://www.abcam.com/index.html?datasheet=97200 (or use the following: http://www.abcam.com/index.html?datasheet=97200).

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Thank you for your patience.

Our customer has responded with the following;

Apologies for the delay, we have been distracted by grant submissions.

I would like a replacement col3 antibody please.


Could you please organise for 1 x FOCR of AB7778.

ANSWER:

Thank you for your reply.

As requested I have sent you a new vial of ab7778 to try out.

Please let me know if there is anything else I can help you with.

Our customer has responded with the following;

responses to your questions are:

1 - Are you still seeing the correct staining pattern, but just weaker than before? Or is there also high background etc?

We see no staining and very low or no background.

2 - When you increased the primary antibody concentration from 1/400 to 1/200, did you see an improvement in the level of staining seen?

No, and we also tried 1:100.

Looking forward to your reply.

ANSWER:

Thank you for your reply.

I believe the best way of dealing with this is to send the customer a new vial of the antibody as if they are getting no signal using a 1/100 dilution, then I don’t think there is much else I can suggest.

If the customer is ok with this plan, or if they would prefer a refund, please let me know.

As always, looking forward to your reply.

DESCRIPTION OF THE PROBLEM very low signal compared to previous batch SAMPLE Mouse Cardiac tissue PRIMARY ANTIBODY COL-3, Abcam rabbit Polyclonal, 1:200, 4c overnight. 1:400 wash-TBSx3 DETECTION METHOD ABC Kit followed by DAB (activated) for 3 mins POSITIVE AND NEGATIVE CONTROLS USED Positive - Previous COL3 batch - positive staining on the same batch of mice cardiac tissue ANTIBODY STORAGE CONDITIONS 4c - Immediate use -20c - long term use FIXATION OF SAMPLE Paraformaldehyde 4% ANTIGEN RETRIEVAL Enzymatic - Trypsin PERMEABILIZATION STEP NA BLOCKING CONDITIONS Goat Serum 10% - 20min SECONDARY ANTIBODY Abcam Goat Polyclonal Secondary Ab to Rabbit IgG (Biotin) (Ab6720), 1:200, 45min incubation. Wash - TBSx3 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? halving dilution of primary Ab (Col3) from 1:400 - 1:200 ADDITIONAL NOTES Recent concentration change by abcam noted 2mg/ml - 1mg/ml. Steps taken halved dilution as above - very poor signal.

ANSWER:

Thank you for contacting Abcam.

I am sorry that you are experiencing some issue with ab7778 in IHC-P on mouse tissue.

I just have a few follow up questions for you:

1 - Are you still seeing the correct staining pattern, but just weaker than before? Or is there also high background etc?

2 - When you increased the primary antibody concentration from 1/400 to 1/200, did you see an improvement in the level of staining seen?

I look forward to your reply and helping you resolve this issue.

LOT NUMBER x ORDER NUMBER z DESCRIPTION OF THE PROBLEM Very Low Signal Compared to the previous Batch SAMPLE Mouse Cardiac Tissue PRIMARY ANTIBODY COL-3 Abcam Rabbit Polyclonal 1:200, 4C overnight 1:400Wash TBS x3 DETECTION METHOD ABC kit followed by DAB(Activated) for 3mins POSITIVE AND NEGATIVE CONTROLS USED Positive - Previous COL3 Batch- Positive Staining on the same batch of mouse cardiac tissues Negetive - Non specific Rabbit IgG ANTIBODY STORAGE CONDITIONS 4C - immediate use -20C long term storage FIXATION OF SAMPLE paraformaldehyde 4% ANTIGEN RETRIEVAL Enzyme trypsin PERMEABILIZATION STEP N/A BLOCKING CONDITIONS Goat Serum 10% 20 mins SECONDARY ANTIBODY Abcam Goat Polyclonal Secondary antibody to Rabbit IgG ( biotin) AB6720, 1:200, 4 mins incubation wash TBS x3 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Halving dilution of primary antibody from 1:400 to 1:200 ADDITIONAL NOTES Recent Concentration change by Abcam noted 2 mg/ml to 1mg/ml. Steps Taken = halve dilution as above = very poor result

ANSWER:

Thank you for contacting us.
I am sorry to hear your customer has been experiencing problems with one of our ab7778 lots. The quality of our products is important to us and I would like to reassure the customer that we investigate all customer complaints. I appreciate the time taken to submit further information to us, and after reading the details provided I would appreciate if your customer can confirm some details of the procedure:
1) Do I understand correctly that with poor results compared to the previous lot you meant there is no signal at all, or at least not a signal that to your experience might be enhanced by increasing the antibody concentration?
2) Could you please clarify the detection method? I.e. could you please confirm the developing time, and that the kit is working well?
Once I have received this confirmation and it turns out that the vial is not giving satisfactory results, we will be pleased to provide a credit note, free of charge replacement or refund.
I look forward to receiving your reply.