Anti-Collagen III antibody (ab7778)

Overview

• Product nameAnti-Collagen III antibodySee all Collagen III primary antibodies ...
• Description
  Rabbit polyclonal to Collagen III
• SpecificityThis type specific collagen antibody only recognizes 3D epitopes. Negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
• Tested applicationsIHC-P, ICC/IF, ICC, ELISA, IHC-Fr, IP, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human
  Predicted to work with: all Mammals
• Immunogen

  Collagen Type III from human and bovine placenta.

• General notesAt least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
• Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
  Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
  Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02461 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 510833 Cow
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 12825 Mouse
  • Entrez Gene: 84032 Rat
  • Omim: 120180 Human
  • SwissProt: P04258 Cow

  • SwissProt: P02461 Human
  • SwissProt: P02461 Human
  • SwissProt: P02461 Human
  • SwissProt: P08121 Mouse
  • SwissProt: P13941 Rat
  • Unigene: 443625 Human
  • Unigene: 249555 Mouse
  • Unigene: 3247 Rat

  see all

• Alternative names
  CO3A1_HUMAN antibodyCOL 3A1 antibodyCOL3A1 antibody

  Collagen alpha 1(III) chain antibodyCollagen alpha-1(III) chain antibodyCollagen III alpha 1 chain precursor antibodyCollagen III alpha 1 polypeptide antibodyCollagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibodyCollagen type III alpha 1 antibodyCollagen type III alpha antibodyEDS4A antibodyEhlers Danlos syndrome type IV, autosomal dominant antibodyFetal collagen antibodyType III collagen antibody

  see all

Anti-Collagen III antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody (ab7778)
  Ab53088 staining Human skin. Staining is localised to the extracellular matrix.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab7778)Image courtesy of Riem Vis PW et al, Tissue Eng Part A. 2010 Apr;16(4):1317-27, Fig 4.
  ab7778 staining Collagen III in Human saphenous vein-derived myofibroblasts by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton-X100. Prior to staining, cells were washed and blocked in a 2% bovine serum albumin, 0.1% saponin (w=v) solution in PBS, and subsequently incubated for 1 hour with the primary antibody in PBS. Nuclei are stained with Hoechst.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)Image courtesy of Rudolf Jung by Abreview.
  ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  ab7778 at 1:400 (45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
• 

  Western blot - Anti-Collagen III antibody (ab7778)
  Anti-Collagen III antibody (ab7778) at 1/1000 dilution + BME
  
  Predicted band size : 138 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  Immunohistochemistry of ab7778. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Protocols

• ELISA to quantify collagen