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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Anti-Collagen III antibody
See all Collagen III products (13) ...
Description
Rabbit polyclonal to Collagen III
Specificity
This type specific collagen antibody only recognizes 3D epitopes. Negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
Tested applications
IHC-P, ICC/IF, ICC, ELISA, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Predicted to work with
all Mammals
Immunogen
Collagen Type III from human and bovine placenta.
General notes
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix.
In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix.
This results in great diversity between collagen types.
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.
For these reasons it is often extremely difficult to generate antibodies with specificities to collagens.
The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.
This preparation results in a native conformation of the protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody (ab7778)
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Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab7778)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab7778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution. (PubMed: 18385800)
ICC: 1/100(PubMed: 19036760)
ELISA: 1/1000 - 1/8000.(This product was assayed against 1.0 ug of Collagen III in a direct ELISA using Peroxidase conjugated Goat anti-Rabbit and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. This product can also be used with Biotin Conjugated Anti-Collagen III (ab6580) in a sandwich ELISA. )
IHC-Fr: 1/50 - 1/200.
IP
IP: 1/5000 - 1/10000.IP: 1/5000 - 1/10000.
WB: 1/5000 - 1/10000. Use under non reducing condition. Detects a band of approximately 300 kDa.(Use under non-denaturing, native conditions.)
Target
Relevance
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Cellular localization
Secreted; extracellular space, extracellular matrix
Alternative names
- COL 3A1 antibody
- COL3A1 antibody
- Collagen alpha 1(III) chain antibody
see all
Database links
- Entrez Gene: 510833 Cow
- Entrez Gene: 1281 Human
- Entrez Gene: 12825 Mouse
- Entrez Gene: 84032 Rat
- Omim: 120180 Human
- SwissProt: P04258 Cow
- SwissProt: P02461 Human
- SwissProt: P08121 Mouse
see all
Anti-Collagen III antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody (ab7778)
Ab53088 staining Human skin. Staining is localised to the extracellular matrix.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - Collagen III antibody (ab7778)
ab7778 staining Collagen III in Human saphenous vein-derived myofibroblasts by Immunocytochemistry/ Immunofluorescence.Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton-X100. Prior to staining, cells were washed and blocked in a 2% bovine serum albumin, 0.1% saponin (w=v) solution in PBS, and subsequently incubated for 1 hour with the primary antibody in PBS. Nuclei are stained with Hoechst.
Image courtesy of Riem Vis PW et al, Tissue Eng Part A. 2010 Apr;16(4):1317-27, Fig 4.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.
Image courtesy of Rudolf Jung by Abreview.
Protocols
References for Anti-Collagen III antibody (ab7778)
This product has been referenced in:
- Akins MLet al. Cervical softening during pregnancy: regulated changes in collagen cross-linking and composition of matricellular proteins in the mouse. Biol Reprod 84:1053-62 (2011). IHC; Mouse.Read more (PubMed: 21248285) »
- Rafael-Fortney JAet al. Early treatment with lisinopril and spironolactone preserves cardiac and skeletal muscle in duchenne muscular dystrophy mice. Circulation 124:582-8 (2011). IHC-Fr; Mouse.Read more (PubMed: 21768542) »
See all 9 publications for this product
Publishing research using ab7778? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"