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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody (ab7778)
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Product Name
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Collagen III antibody
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See all Collagen III antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Collagen III
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Immunogen
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Collagen Type III from human and bovine placenta.
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Mamm
(Due to sequence homology)
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Specificity
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This type specific collagen antibody only recognizes 3D epitopes.
Negligible cross-reactivity with Type I, II, IV, V or VI collagens.
Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000 - 1/8000.
ICC: 1/100 (PMID 19036760).
ICC/IF: Use at an assay dependent dilution (PMID 18385800).
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/50 - 1/200.
IP: 1/5000 - 1/10000.
WB: 1/5000 - 1/10000. Use under non-denaturing, non-reducing conditions. Detects a band of approximately 300 kDa.
This product was assayed against 1.0 ug of Collagen III in a direct ELISA using Peroxidase conjugated Goat anti-Rabbit and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. This product can also be used with Biotin Conjugated Anti-Collagen III (ab6580) in a sandwich ELISA.
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted; extracellular space, extracellular matrix
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Research areas
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Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Relevance
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Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix.
In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix.
This results in great diversity between collagen types.
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.
For these reasons it is often extremely difficult to generate antibodies with specificities to collagens.
The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.
This preparation results in a native conformation of the protein.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Collagen III antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Collagen III antibody images, references, products related to ab7778 and other tools.
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Collagen III antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Collagen III antibody (ab7778)
Ab53088 staining Human skin. Staining is localised to the extracellular matrix.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for Collagen III antibody (ab7778)
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This product has been used in: (two most recent references)
Artaza JN & Norris KC Vitamin D reduces the expression of collagen and key profibrotic factors by inducing an antifibrotic phenotype in mesenchymal multipotent cells. J Endocrinol 200:207-21 (2009). ICC; Mouse. PubMed: 19036760
Umar S et al. Allogenic stem cell therapy improves right ventricular function by improving lung pathology in rats with pulmonary hypertension. Am J Physiol Heart Circ Physiol 297:H1606-16 (2009). IHC-P; Rat. PubMed: 19783775
See all 5 publication references for this product.
If you publish research using ab7778 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Collagen III
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