Anti-Collagen III antibody (ab83829)

Overview

• Product nameAnti-Collagen III antibodySee all Collagen III primary antibodies ...
• Description
  Rabbit polyclonal to Collagen III
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rhesus monkey
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Collagen III.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab101725.)

• Positive controlThis antibody gave a positive signal in the following lysates: Skin (Human) Tissue Lysate; Small Intestine (Human) Tissue Lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
• Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
  Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
  Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02461 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Omim: 120180 Human
  • SwissProt: P02461 Human
  • SwissProt: P02461 Human
  • SwissProt: P02461 Human
  • Unigene: 443625 Human

• Alternative names
  CO3A1_HUMAN antibodyCOL 3A1 antibodyCOL3A1 antibody

  Collagen alpha 1(III) chain antibodyCollagen alpha-1(III) chain antibodyCollagen III alpha 1 chain precursor antibodyCollagen III alpha 1 polypeptide antibodyCollagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibodyCollagen type III alpha 1 antibodyCollagen type III alpha antibodyEDS4A antibodyEhlers Danlos syndrome type IV, autosomal dominant antibodyFetal collagen antibodyType III collagen antibody

  see all

Anti-Collagen III antibody images

• 

  Western blot - Collagen III antibody (ab83829)
  All lanes : Anti-Collagen III antibody (ab83829) at 1 µg/ml
  
  Lane 1 : Skin (Human) Tissue Lysate - adult normal tissue (ab30166)
  Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 138 kDa
  Observed band size : 138 kDa
  Additional bands at : 200 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes