Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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What is the Peptide region? |
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ANSWER: |
I realized that I may not have written down the ID number of the product you were interested in correctly. Could you please send me the correct abID number and I will be able to complete you request. |
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what is the cross reactivity of ab7533 and ab7535 with collagen type I? |
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ANSWER: |
These products have been prepared from human placenta and are chromatographically and immunologically pure. They are free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reaction with our anti-Collagen antibodies I, II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA) apart from the expect cross reaction with the collagen type i.e ab7535 cross reacts with the anti-collagen III antibody, and ab7533 cross reacts with the anti-collagen I antibody. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : plus ab7535 reduced and denatured
Lane 2 : plus ab7535 native
Secondary
Goat anti-mouse-HRP at 1/2000 dilution
The protein can be used reduced + denaturated (band size ~130 kDa)
or native (two bands ~ 130kDa and ~300kDa)
The native protein gives a brighter signal.PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.
Image courtesy of Michaela Leyh by Abreview.
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