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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Collagen III protein
See all Collagen III products (4) ...
Protein description
Full length native protein (purified) (Human placenta)
Properties
Purification notes
This product has been prepared from human placenta and is chromatographically and immunologically pure.
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.5M Acetic Acid. pH 4.5
Concentration
Concentration information loading...
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Western blot - Collagen III protein (ab7535)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent concentration. (This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.)
ELISA: Use at an assay dependent concentration.
Protein info
Relevance
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Cellular localization
Secreted; extracellular space, extracellular matrix
Collagen III protein images:
Western blot - Collagen III protein (ab7535)
All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : plus ab7535 reduced and denatured
Lane 2 : plus ab7535 native
Secondary
Goat anti-mouse-HRP at 1/2000 dilution
The protein can be used reduced + denaturated (band size ~130 kDa)
or native (two bands ~ 130kDa and ~300kDa)
The native protein gives a brighter signal.PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.
Image courtesy of Michaela Leyh by Abreview.
References for Collagen III protein (ab7535)
ab7535 has not yet been referenced specifically in any publications.
Publishing research using ab7535? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"