Anti-Collagen IV alpha 1 antibody (ab189408)
Key features and details
- Rabbit polyclonal to Collagen IV alpha 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Collagen IV alpha 1 antibody
See all Collagen IV alpha 1 primary antibodies -
Description
Rabbit polyclonal to Collagen IV alpha 1 -
Host species
Rabbit -
Specificity
ab189408 recognizes extracellular - basement membranes. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Full length native protein (purified) corresponding to Human Collagen IV alpha 1. Isolated from Human placenta
Database link: P02462 -
Positive control
- Human kidney tissue, glomerulus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/75 - 1/100.
Sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. |
Notes |
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IHC-P
1/75 - 1/100. Sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. |
Target
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Relevance
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. -
Database links
- Entrez Gene: 282191 Cow
- Entrez Gene: 1282 Human
- Entrez Gene: 100515336 Pig
- Entrez Gene: 290905 Rat
- Omim: 120130 Human
- SwissProt: Q7SIB2 Cow
- SwissProt: P02462 Human
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Alternative names
- Arresten antibody
- COL4A1 NC1 domain antibody
- Collagen alpha-1(IV) chain antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab189408 has been referenced in 2 publications.
- Chen L et al. Morphologic, Biomechanical, and Compositional Features of the Internal Limiting Membrane in Pathologic Myopic Foveoschisis. Invest Ophthalmol Vis Sci 59:5569-5578 (2018). PubMed: 30480705
- Kriz W et al. Accumulation of worn-out GBM material substantially contributes to mesangial matrix expansion in diabetic nephropathy. Am J Physiol Renal Physiol 312:F1101-F1111 (2017). PubMed: 28228399