Overview

  • Product nameAnti-Collagen IV antibody [1043]See all Collagen IV primary antibodies ...
  • Description
    Mouse monoclonal [1043] to Collagen IV
  • Specificityab86042 recognizes the extracellular basement membranes.
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC, Flow Cyt more details
  • Species reactivity
    Reacts with: Cow, Human, Pig
  • Immunogen

    Full length native protein, purified from Human placenta

  • General notes


    In IHC, recognizes the extracellular – basement membranes.

Properties

Applications

Our Abpromise guarantee covers the use of ab86042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/100 - 1/1000. Use under non reducing condition. Predicted molecular weight: 160 kDa.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC 1/50 - 1/100. Methanol fixed.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Tissue specificityHighly expressed in placenta.
  • Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • Sequence similaritiesBelongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • Post-translational
    modifications
    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Target information above from: UniProt accession P02462 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Arresten antibody
    • CO4A1_HUMAN antibody
    • COL4A1 antibody
    • COL4A1 NC1 domain antibody
    • COL4A2 antibody
    • COL4A3 antibody
    • COL4A4 antibody
    • COL4A5 antibody
    • collagen alpha-1(IV) chain antibody
    • Collagen IV Alpha 1 Polypeptide antibody
    • Collagen IV Alpha 2 Polypeptide antibody
    • Collagen Of Basement Membrane Alpha 1 Chain antibody
    • Collagen Of Basement Membrane Alpha 2 Chain antibody
    • Collagen Type IV Alpha 1 antibody
    • Collagen Type IV Alpha 2 antibody
    • Collagen Type IV Alpha 3 antibody
    • Collagen Type IV Alpha 4 antibody
    • Collagen Type IV Alpha 5 antibody
    see all

Anti-Collagen IV antibody [1043] images

  • Immunohistochemistry on frozen section of Human colon
  • Immunohistochemistry on paraffin section of Human skin

References for Anti-Collagen IV antibody [1043] (ab86042)

This product has been referenced in:
  • Havenith MG  et al. Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 87:123-8 (1987). Read more (PubMed: 3305430) »

See 1 Publication for this product

Product Wall

Your interpretation of the information is correct. It has been found with this antibody that it can be used to detect the denatured protein but not with a reducing agent present. This is illustrated in the following reference:

Havenith MG et a...

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Thank you for contacting us.

This antibody has not been tested in rat. A BLAST search of the immunogen showed 84% homology with the rat sequence, so it is likely to cross-react.

I hope this information is helpful to you. Please do...

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Thank you for clarifying that you would prefer to do IHC on paraffin tissue sections rather than frozen tissue sections. For both of these applications I would indeed suggest checking the biocompare website. Ideally you would need a mouse anti-rabbit c...

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I am sorry, I was under the impression that you wanted to do immunohistochemistry on rabbit tissue. If this is not the case, what application would you like to use the anti-collagen IV antibody in?

With regards to finding an antibody which is...

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As previously discussed with you, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. It is regrettably very difficult to suggest antibodies to custome...

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Thank you for contacting us in regards to anti-Collagen IV antibodies.

As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or an...

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\It is recommended that 0.1 mM CaCl2 and 0.1 mM MgCl2 should be included in the primary antibody incubation buffer. If the initial antibody incubation buffer did not contain 0.1 mM CaCl2 and 0.1 mM MgCl2, I think it is worthwhile to try the staining on...

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