Overview

  • Product nameAnti-Collagen IV antibody [C IV 22]
    See all Collagen IV primary antibodies
  • Description
    Mouse monoclonal [C IV 22] to Collagen IV
  • SpecificityDoes not react with other types of collagen.
  • Tested applicationsIHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • EpitopeThe antibody recognizes a conformational epitope on a helical part of native collagen IV and loses its reactivity on denaturing the protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab15633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. The antigen is a helical determinant of the native type IV collagen. Therefore clone CIV 22 is detects only the native form. It is stable to formaldehyde fixation and paraffin embedding. The antigen can be detected in all basement membranes with the exception of the basement membrane of the corneal epithelium.
IHC-Fr Use at an assay dependent dilution. PubMed: 6209713

Target

  • FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Tissue specificityHighly expressed in placenta.
  • Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • Sequence similaritiesBelongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • Post-translational
    modifications
    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arresten antibody
    • Canstatin antibody
    • CO4A1_HUMAN antibody
    • COL4A1 antibody
    • COL4A1 NC1 domain antibody
    • COL4A2 antibody
    • COL4A3 antibody
    • COL4A4 antibody
    • COL4A5 antibody
    • Collagen Alpha 1(IV) Chain antibody
    • Collagen Alpha 2(IV) Chain antibody
    • collagen alpha-1(IV) chain antibody
    • Collagen IV Alpha 1 Polypeptide antibody
    • Collagen IV Alpha 2 Polypeptide antibody
    • Collagen Of Basement Membrane Alpha 1 Chain antibody
    • Collagen Of Basement Membrane Alpha 2 Chain antibody
    • Collagen Type IV Alpha 1 antibody
    • Collagen Type IV Alpha 2 antibody
    • Collagen Type IV Alpha 3 antibody
    • Collagen Type IV Alpha 4 antibody
    • Collagen Type IV Alpha 5 antibody
    • DKFZp686I14213 antibody
    • FLJ22259 antibody
    see all

References for Anti-Collagen IV antibody [C IV 22] (ab15633)

This product has been referenced in:
  • Pigors M  et al. Lack of plakoglobin leads to lethal congenital epidermolysis bullosa: a novel clinico-genetic entity. Hum Mol Genet 20:1811-9 (2011). IHC-Fr ; Human . Read more (PubMed: 21320868) »
  • Odermatt BF  et al. Monoclonal antibodies to human type IV collagen: useful reagents to demonstrate the heterotrimeric nature of the molecule. Proc Natl Acad Sci U S A 81:7343-7 (1984). IHC-Fr ; Human . Read more (PubMed: 6209713) »

See all 2 Publications for this product

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The antibody recognizes a conformational epitope on a helical part of native collagen IV and loses its reactivity on denaturing the collagen IV protein.

The exact epitope sequence is not determined for thi...

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Thank you for your patience. I have heard back from the lab regarding ab15633: The antigen retrieval step was performed with proteinase K, 7 min incubation at RT. The specific IHC protocol for paraffin sections is as follows: Protocol with form...

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Thank you for your reply and the additional information. This is very helpful to us. Please let me know what species the placenta tissue was from as well as your order or PO number. According to your protocol information, you have tried a sever...

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Thank you for contacting us. I  am sorry to hear that you are having problems with these antibodies. Below I have collected the information for each antibody. ab6308: Sections were PFA fixed and subjected to heat mediated antigen retrieval in cit...

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