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Full length native protein (purified) (Human).
Our Abpromise guarantee covers the use of ab15633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use a concentration of 0.5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. The antigen is a helical determinant of the native type IV collagen. Therefore clone CIV 22 is detects only the native form. It is stable to formaldehyde fixation and paraffin embedding. The antigen can be detected in all basement membranes with the exception of the basement membrane of the corneal epithelium.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 6209713|
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