Anti-Collagen IV antibody [C IV 22] (ab15633)

Overview

• Product nameAnti-Collagen IV antibody [C IV 22]See all Collagen IV primary antibodies ...
• Description
  Mouse monoclonal [C IV 22] to Collagen IV
• SpecificityDoes not react with other types of collagen.
• Tested applicationsIHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Cow, Human
  
• Immunogen

  Full length native protein (purified) (Human).

• EpitopeThe antibody recognizes a conformational epitope on a helical part of native collagen IV and loses its reactivity on denaturing the protein.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal / Merthiolate
  Constituents: PBS, 10mg/ml BSA. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone numberC IV 22
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Basal Lamina

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
  Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
• Tissue specificityHighly expressed in placenta.
• Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
  Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
  Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
• Sequence similaritiesBelongs to the type IV collagen family.
  Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
• DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
• Post-translational
  modificationsLysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
  Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
  The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
  Proteolytic processing produces the C-terminal NC1 peptide, arresten.
• Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.

Target information above from: UniProt accession P02462 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282191 Cow
  • Entrez Gene: 508632 Cow
  • Entrez Gene: 317711 Cow
  • Entrez Gene: 407107 Cow
  • Entrez Gene: 511602 Cow
  • Entrez Gene: 1284 Human
  • Entrez Gene: 1285 Human
  • Entrez Gene: 1287 Human

  • Entrez Gene: 1286 Human
  • Entrez Gene: 1282 Human
  • Omim: 120130 Human
  • Omim: 120090 Human
  • Omim: 120070 Human
  • Omim: 303630 Human
  • Omim: 120131 Human
  • SwissProt: P02453 Cow
  • SwissProt: P08572 Human
  • SwissProt: Q01955 Human
  • SwissProt: P29400 Human
  • SwissProt: P53420 Human
  • SwissProt: P02462 Human
  • Unigene: 570065 Human
  • Unigene: 591645 Human
  • Unigene: 369089 Human
  • Unigene: 17441 Human

  see all

• Alternative names
  Arresten antibodyCanstatin antibodyCO4A1_HUMAN antibody

  COL4A1 antibodyCOL4A1 NC1 domain antibodyCOL4A2 antibodyCOL4A3 antibodyCOL4A4 antibodyCOL4A5 antibodyCollagen Alpha 1(IV) Chain antibodyCollagen Alpha 2(IV) Chain antibodycollagen alpha-1(IV) chain antibodyCollagen IV Alpha 1 Polypeptide antibodyCollagen IV Alpha 2 Polypeptide antibodyCollagen Of Basement Membrane Alpha 1 Chain antibodyCollagen Of Basement Membrane Alpha 2 Chain antibodyCollagen Type IV Alpha 1 antibodyCollagen Type IV Alpha 2 antibodyCollagen Type IV Alpha 3 antibodyCollagen Type IV Alpha 4 antibodyCollagen Type IV Alpha 5 antibodyDKFZp686I14213 antibodyFLJ22259 antibody

  see all