Directly conjugated antibodies for immunofluorescence

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  • Product nameAnti-Collagen IV antibody [COL-94]
    See all Collagen IV primary antibodies
  • Description
    Mouse monoclonal [COL-94] to Collagen IV
  • SpecificitySpecifically recognizes native, undenatured, human collagen type IV . Shows no cross reactivity with collagen types I, II, III, V, VI, VII, human vitronectin, fibronectin, or chondroitin sulfate A. B and C. No reactivity with denatured or denatured-reduced collagen in immunoblots.
  • Tested applicationsWB, ELISA, IHC-FoFr, IHC-P, Dot Blot, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates

    Does not react with

    Rat, Sheep, Goat, Chicken, Cat
  • Immunogen

    Full length native protein (purified) (Human).

  • EpitopeRecognises an epitope located on the alpha 1 and/or alpha 2 chains of human collage type IV.
  • Positive control
    • Human skin tissue.
  • General notes

    Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.



Our Abpromise guarantee covers the use of ab6311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    ICC/IF: 1/200.
    IHC-P: 1/300 (PMID 18843029).
    IHC-FoFr: 1/500.
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
      Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
    • Tissue specificityHighly expressed in placenta.
    • Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
      Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
      Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
    • Sequence similaritiesBelongs to the type IV collagen family.
      Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
    • DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
    • Post-translational
      Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
      The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
      Proteolytic processing produces the C-terminal NC1 peptide, arresten.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Arresten antibody
      • Canstatin antibody
      • CO4A1_HUMAN antibody
      • COL4A1 antibody
      • COL4A1 NC1 domain antibody
      • COL4A2 antibody
      • COL4A3 antibody
      • COL4A4 antibody
      • COL4A5 antibody
      • Collagen Alpha 1(IV) Chain antibody
      • Collagen Alpha 2(IV) Chain antibody
      • collagen alpha-1(IV) chain antibody
      • Collagen IV Alpha 1 Polypeptide antibody
      • Collagen IV Alpha 2 Polypeptide antibody
      • Collagen Of Basement Membrane Alpha 1 Chain antibody
      • Collagen Of Basement Membrane Alpha 2 Chain antibody
      • Collagen Type IV Alpha 1 antibody
      • Collagen Type IV Alpha 2 antibody
      • Collagen Type IV Alpha 3 antibody
      • Collagen Type IV Alpha 4 antibody
      • Collagen Type IV Alpha 5 antibody
      • Collagen Type IV antibody
      • DKFZp686I14213 antibody
      • FLJ22259 antibody
      see all

    Anti-Collagen IV antibody [COL-94] images

    • Immunofluorescence analysis of Human endothelial cells, staining Collagen IV with ab6311.
      Cells were fixed with formaldehyde and permeabilized with acetone. Samples were incubated with primary antibody (0.5 µg/ml in diluent) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (5 µg/ml) (ab150113) was used as the secondary antibody.

      See Abreview

    References for Anti-Collagen IV antibody [COL-94] (ab6311)

    This product has been referenced in:
    • Marino D  et al. Bioengineering dermo-epidermal skin grafts with blood and lymphatic capillaries. Sci Transl Med 6:221ra14 (2014). Read more (PubMed: 24477001) »
    • Forget A  et al. Polysaccharide hydrogels with tunable stiffness and provasculogenic properties via a-helix to ß-sheet switch in secondary structure. Proc Natl Acad Sci U S A 110:12887-92 (2013). Read more (PubMed: 23886665) »

    See all 6 Publications for this product

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    Thank you for contacting us.

    Thank you for clarifying that you would prefer to do IHC on paraffin tissue sections rather than frozen tissue sections. For both of these applications I would indeed suggest checking the biocompare website. Idea...

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    Thank you for contacting us.

    I am sorry, I was under the impression that you wanted to do immunohistochemistry on rabbit tissue. If this is not the case, what application would you like to use the anti-collagen IV antibody in?


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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Endothelial in Matrigel)
    Specification Endothelial in Matrigel
    Fixative Formaldehyde
    Permeabilization Yes - Acetone

    Abcam user community

    Verified customer

    Submitted Sep 25 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (skin)
    Specification skin
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Trititon X-100
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 17 2012

    Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab6311 in mouse.

    Expiration date:13/11/11

    This code will give you: 1 free primary antibody before th...

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    Thank you for your reply and further enquiry which has been forwarded to me as my colleague Karin is currently away from the office.

    As Karin previously discussed with you, ab6311, has been shown to not cross react with a number of different...

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    Thank you for contacting us in regards to anti-Collagen IV antibodies.

    As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or an...

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    Thank you for contacting us.

    I am sorry to hear you have been experiencing problems with ab6311. As you have had success with ab6586, I would be happy to replace the product. Please could you confirm the lot number and date of order so I can...

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    Thank you for your enquiry regarding ab6311 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

    Though you have kindly provided some deta...

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    Vielen Dank für Ihren Anruf. Es tut mir leid, dass Sie wieder Probleme mit dem Collagen-Antikörper ab6311 hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung mit ab86042 für Sie in Auftrag gegeben. Sie hat die Referenznummer ...

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