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Anti-Collagen IV antibody [PHM-12] (ab49213)

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Overview

  • Product nameAnti-Collagen IV antibody [PHM-12]See all Collagen IV primary antibodies ...
  • Description
    Mouse monoclonal [PHM-12] to Collagen IV
  • SpecificityIn kidney, NCL-COLL-IV reacts with glomerular and tubular basement membranes and also mesangial cells and matrix within the glomerulus. The antibody reacts with the basal lamina of capillaries as well as basement membrane structures in all organs examined.
  • Tested applicationsIHC-Fr, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human glomeruli

  • Positive controlKidney tissue; basement membranes.

Properties

Applications

Our Abpromise guarantee covers the use of ab49213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr
IHC-P
  • Application notesIHC-Fr: 1/100 - 1/200.
    IHC-P: 1/100 - 1/200. Heat mediated antigen retrieval and 30 seconds of trypsin digestion are recommended before commencing with IHC staining protocol. Use 60 minutes primary antibody incubation at 25oC and standard ABC technique.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
      Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
    • Tissue specificityHighly expressed in placenta.
    • Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
      Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
      Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
    • Sequence similaritiesBelongs to the type IV collagen family.
      Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
    • DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
    • Post-translational
      modifications      Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
      The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
      Proteolytic processing produces the C-terminal NC1 peptide, arresten.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.

      • Target information above from: UniProt accession P02462 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Arresten antibodyCanstatin antibodyCO4A1_HUMAN antibody
        COL4A1 antibodyCOL4A1 NC1 domain antibodyCOL4A2 antibodyCOL4A3 antibodyCOL4A4 antibodyCOL4A5 antibodyCollagen Alpha 1(IV) Chain antibodyCollagen Alpha 2(IV) Chain antibodycollagen alpha-1(IV) chain antibodyCollagen IV Alpha 1 Polypeptide antibodyCollagen IV Alpha 2 Polypeptide antibodyCollagen Of Basement Membrane Alpha 1 Chain antibodyCollagen Of Basement Membrane Alpha 2 Chain antibodyCollagen Type IV Alpha 1 antibodyCollagen Type IV Alpha 2 antibodyCollagen Type IV Alpha 3 antibodyCollagen Type IV Alpha 4 antibodyCollagen Type IV Alpha 5 antibodyDKFZp686I14213 antibodyFLJ22259 antibody
      see all

    References for Anti-Collagen IV antibody [PHM-12] (ab49213)

    ab49213 has not yet been referenced specifically in any publications.

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