Overview

  • Product nameAnti-Collagen IV antibodySee all Collagen IV primary antibodies ...
  • Description
    Rabbit polyclonal to Collagen IV
  • Tested applicationsIHC-Fr, IHC-P, IHC-FoFr, WB, IHC-FrFl, ICC/IF, RIA, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Full length native protein (extracted and purified from tumor tissues) (Mouse).

  • Positive control
    • Mouse brain

Properties

Applications

Our Abpromise guarantee covers the use of ab19808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr
IHC-P
IHC-FoFr
WB
IHC-FrFl
ICC/IF
RIA
ELISA
  • Application notesELISA: 1/200.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/500.
    IHC-Fr: 1/400.
    IHC-FoFr: Use at an assay dependent dilution (PMID 19762493).
    RIA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
      Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
    • Tissue specificityHighly expressed in placenta.
    • Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
      Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
      Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
    • Sequence similaritiesBelongs to the type IV collagen family.
      Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
    • DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
    • Post-translational
      modifications
      Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
      The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
      Proteolytic processing produces the C-terminal NC1 peptide, arresten.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
    • Target information above from: UniProt accession P02462 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Arresten antibody
      • Canstatin antibody
      • CO4A1_HUMAN antibody
      • COL4A1 antibody
      • COL4A1 NC1 domain antibody
      • COL4A2 antibody
      • COL4A3 antibody
      • COL4A4 antibody
      • COL4A5 antibody
      • Collagen Alpha 1(IV) Chain antibody
      • Collagen Alpha 2(IV) Chain antibody
      • collagen alpha-1(IV) chain antibody
      • Collagen IV Alpha 1 Polypeptide antibody
      • Collagen IV Alpha 2 Polypeptide antibody
      • Collagen Of Basement Membrane Alpha 1 Chain antibody
      • Collagen Of Basement Membrane Alpha 2 Chain antibody
      • Collagen Type IV Alpha 1 antibody
      • Collagen Type IV Alpha 2 antibody
      • Collagen Type IV Alpha 3 antibody
      • Collagen Type IV Alpha 4 antibody
      • Collagen Type IV Alpha 5 antibody
      • DKFZp686I14213 antibody
      • FLJ22259 antibody
      see all

    Anti-Collagen IV antibody images

    • ab19808 at a 1/200 dilution staining mouse mammary gland tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected with an Alexa Fluor® 488 conjuaged anti-rabbit antibody.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 26 January 2006.

      See Abreview

    • ab19808 staining Collagen IV in mouse tooth tissue section by Immunohistochemistry (Frozen sections). Tissue samples were blocked with 1% BSA for 20 minutes at 200C and incubated with undiluted primary antibody for 1 hour at 200C. An Alexa Fluor®594-conjugated chicken polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution. Red colour in the image represents staining of CoIlagen-IV and the green is for CD31, yellow for ED16+6, which were purchased from different sources.

      See Abreview

    • ab19808 staining Collagen IV in murine brain tissue/ human xenograft tissue by Immunohistochemistry.
      Tissue was fixed in AFA (alcohol-formal-acetate) and a heat mediated antigen retrieval step was performed using Tris-EDTA pH 9. Samples were then blocked using 3% BSA for 30 minutes at 20°C and then incubated with ab19808 at a 1/500 dilution for 1 hour and 30 minutes. The secondary used was an undiluted HRP-conjugated goat polyclonal.

      Left side: normal mouse brain
      Right side: human glioblastoma xenograft

      See Abreview

    • ab19808 staining Collagen IV in mouse brain cells (ab30149) by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with formaldehyde and blocked with 0.25% TNB for 30 minutes at 22°C. Samples were incubated with primary antibody 1/250 in TNB for 18 hours at 22°C. A Biotin-conjugated Goat polyclonal to rabbit IgG (ab6720), dilution 1/500, was used as secondary antibody.

      See Abreview

    References for Anti-Collagen IV antibody (ab19808)

    This product has been referenced in:
    • Whiteus C  et al. Perturbed neural activity disrupts cerebral angiogenesis during a postnatal critical period. Nature 505:407-11 (2014). IHC ; Mouse . Read more (PubMed: 24305053) »
    • Ishikawa H  et al. Vasculogenesis in experimental stroke after human cerebral endothelial cell transplantation. Stroke 44:3473-81 (2013). Rat . Read more (PubMed: 24130140) »

    See all 31 Publications for this product

    Product Wall

    Thank you for your recent telephone enquiry. i am sorry it has taken some time to obtain this information for you from the originator.

    I am sorry to confirm that in this particular case, the buffer components are proprietary. We are able to ...

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    Thank you for contacting us.

    There isn’t a specific protocol for performing WB with this antibody. We recommend using reducing and denaturing conditions unless otherwise stated on the datasheet. For this particular antibody there isn&rs...

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    Thanks for your call today and for your questions about our collagen IV antibodies.
    As we discussed, I would still recommend trying ab6586 with your standard Western blotting conditions. We recommend non-denatured conditions because the affinity o...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (brain)
    Specification brain
    Fixative Alcool-Formol-Acetate (AFA)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 23 2011

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Mouse brain cells)
    Specification Mouse brain cells
    Fixative Alcool-Formol-Acetate (AFA)
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 15 2011

    Merci de votre réponse. La recette pour le TBS 10x est la suivante : 24.23 g Trizma HCl 80.06 g NaCl Mix in 800 ml ultra pure water. pH to 7.6 with pure HCl. Top up to 1 L. Ceci doit être dilué 10x pour obtenir du TBS 1...

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    Merci pour nous avoir contacté. Je suis désolée que vous avez des problèmes avec un de nos anticorps. Comme discuté, j'ai donc contacté le laboratoire pour avoir plus d'information sur le protocole et le démasquage utilisé avec cet anticorps. ...

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    Thanks for your enquiry. ab19808 is diluted in deionized water. I hope this is helpful. Please contact us again if you have any further questions.

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Kidney)
    Specification Kidney
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% Triton X-100
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 09 2010

    Application Immunohistochemistry - Free Floating
    Sample Mouse Tissue sections (brain blood vessels)
    Specification brain blood vessels
    Username

    Ms. Christina Whiteus

    Verified customer

    Submitted Oct 20 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"