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Full length native protein (extracted and purified from tumor tissues) (Mouse).
Our Abpromise guarantee covers the use of ab19808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Permeabilisation with ~0.2% Triton is recommended
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19762493|
|WB||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22253831|
|RIA||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration. PubMed: 24353059|
ab19808 at a 1/200 dilution staining mouse mammary gland tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected with an Alexa Fluor® 488 conjuaged anti-rabbit antibody.
This image is courtesy of an Abreview submitted by an anonymous researcher on 26 January 2006.
ab19808 staining Collagen IV in mouse brain cells (ab30149) by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with formaldehyde and blocked with 0.25% TNB for 30 minutes at 22°C. Samples were incubated with primary antibody 1/250 in TNB for 18 hours at 22°C. A Biotin-conjugated Goat polyclonal to rabbit IgG (ab6720), dilution 1/500, was used as secondary antibody.
ab19808 staining Collagen IV in mouse tooth tissue section by Immunohistochemistry (Frozen sections). Tissue samples were blocked with 1% BSA for 20 minutes at 200C and incubated with undiluted primary antibody for 1 hour at 200C. An Alexa Fluor®594-conjugated chicken polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution. Red colour in the image represents staining of CoIlagen-IV and the green is for CD31, yellow for ED16+6, which were purchased from different sources.
ab19808 staining Collagen IV in murine brain tissue/ human xenograft tissue by Immunohistochemistry. Tissue was fixed in AFA (alcohol-formal-acetate) and a heat mediated antigen retrieval step was performed using Tris-EDTA pH 9. Samples were then blocked using 3% BSA for 30 minutes at 20°C and then incubated with ab19808 at a 1/500 dilution for 1 hour and 30 minutes. The secondary used was an undiluted HRP-conjugated goat polyclonal. Left side: normal mouse brainRight side: human glioblastoma xenograft