Anti-Collagen IV antibody (ab19808)

Overview

• Product nameAnti-Collagen IV antibodySee all Collagen IV primary antibodies ...
• Description
  Rabbit polyclonal to Collagen IV
• SpecificityRecognises Mouse Collagen Type IV (100% by RIA). Does not react with Mouse Collagen Types I or III, Human Collagen Type IV or V, Mouse Fibronectin or Mouse Laminin (<0.1% by RIA). We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
• Tested applicationsIHC-Fr, IHC-P, IHC-FoFr, WB, IHC-FrFl, ICC/IF, RIA, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat
  
• Immunogen

  Full length native protein (extracted and purified from tumor tissues) (Mouse).

Properties

• FormLiquid
• Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesIon exchange chromatography (DEAE-Trisacryl).
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Basal Lamina

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
  Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
• Tissue specificityHighly expressed in placenta.
• Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
  Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
  Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
• Sequence similaritiesBelongs to the type IV collagen family.
  Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
• DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
• Post-translational
  modificationsLysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
  Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
  The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
  Proteolytic processing produces the C-terminal NC1 peptide, arresten.
• Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.

Target information above from: UniProt accession P02462 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 12827 Mouse
  • Entrez Gene: 12828 Mouse
  • Entrez Gene: 12830 Mouse
  • Entrez Gene: 12829 Mouse
  • Entrez Gene: 12826 Mouse
  • Entrez Gene: 306628 Rat
  • Entrez Gene: 363265 Rat
  • Entrez Gene: 290905 Rat

  • Entrez Gene: 301562 Rat
  • Entrez Gene: 363457 Rat
  • SwissProt: P08122 Mouse
  • SwissProt: Q9QZS0 Mouse
  • SwissProt: Q9QZR9 Mouse
  • SwissProt: P02463 Mouse
  • Unigene: 738 Mouse
  • Unigene: 53801 Rat
  • Unigene: 2237 Rat
  • Unigene: 121139 Rat
  • Unigene: 22075 Rat
  • Unigene: 17094 Rat

  see all

• Alternative names
  Arresten antibodyCanstatin antibodyCO4A1_HUMAN antibody

  COL4A1 antibodyCOL4A1 NC1 domain antibodyCOL4A2 antibodyCOL4A3 antibodyCOL4A4 antibodyCOL4A5 antibodyCollagen Alpha 1(IV) Chain antibodyCollagen Alpha 2(IV) Chain antibodycollagen alpha-1(IV) chain antibodyCollagen IV Alpha 1 Polypeptide antibodyCollagen IV Alpha 2 Polypeptide antibodyCollagen Of Basement Membrane Alpha 1 Chain antibodyCollagen Of Basement Membrane Alpha 2 Chain antibodyCollagen Type IV Alpha 1 antibodyCollagen Type IV Alpha 2 antibodyCollagen Type IV Alpha 3 antibodyCollagen Type IV Alpha 4 antibodyCollagen Type IV Alpha 5 antibodyDKFZp686I14213 antibodyFLJ22259 antibody

  see all

Anti-Collagen IV antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - Collagen IV antibody (ab19808)

  ab19808 at a 1/200 dilution staining mouse mammary gland tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected with an Alexa Fluor® 488 conjuaged anti-rabbit antibody.

  This image is courtesy of an Abreview submitted by an anonymous researcher on 26 January 2006.

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• 

  Immunocytochemistry/ Immunofluorescence - Collagen IV antibody (ab19808)This image is courtesy of an anonymous Abreview
  ab19808 staining Collagen IV in mouse brain cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with formaldehyde and blocked with 0.25% TNB for 30 minutes at 22°C. Samples were incubated with primary antibody 1/250 in TNB for 18 hours at 22°C. A Biotin-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Collagen IV antibody (ab19808)This image is a courtesy of Adnane Nait Lechguer

  ab19808 staining Collagen IV in mouse tooth tissue section by Immunohistochemistry (Frozen sections). Tissue samples were blocked with 1% BSA for 20 minutes at 20⁰C and incubated with undiluted primary antibody for 1 hour at 20⁰C. An Alexa Fluor^®594-conjugated chicken polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution. Red colour in the image represents staining of CoIlagen-IV and the green is for CD31, yellow for ED16+6, which were purchased from different sources.

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• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab19808)Image courtesy of an anonymous Abreview.
  ab19808 staining Collagen IV in murine brain tissue/ human xenograft tissue by Immunohistochemistry.
  
  Tissue was fixed in AFA (alcohol-formal-acetate) and a heat mediated antigen retrieval step was performed using Tris-EDTA pH 9. Samples were then blocked using 3% BSA for 30 minutes at 20°C and then incubated with ab19808 at a 1/500 dilution for 1 hour and 30 minutes. The secondary used was an undiluted HRP-conjugated goat polyclonal.
  
  Left side: normal mouse brain
  Right side: human glioblastoma xenograft

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