Anti-Collagen IV antibody (ab21295)

Overview

• Product nameAnti-Collagen IV antibodySee all Collagen IV primary antibodies ...
• Description
  Rabbit polyclonal to Collagen IV
• SpecificityRecognises: Human collagen type IV 100% Human collagen types I, II <0.1% Human collagen type III, V <1.0% Human Fibronectin <0.1% Mouse Laminin <0.1%
• Tested applicationsIHC-Fr, ICC/IF, ELISA, RIA, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length native protein (purified): Collagen type IV extracted and purified from human placenta

• Positive controlIHC-P: human placenta FFPE tissue sections.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Concentration information loading...
• PurityIgG fraction
• Purification notesPurified by Ion exchange chromatography (DEAE-Trisacryl)
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

  • Signal Transduction
  • Cytoskeleton / ECM
  • Basal Lamina

Applications

Target

• FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
  Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
• Tissue specificityHighly expressed in placenta.
• Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
  Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
  Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
• Sequence similaritiesBelongs to the type IV collagen family.
  Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
• DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
• Post-translational
  modificationsLysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
  Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
  The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
  Proteolytic processing produces the C-terminal NC1 peptide, arresten.
• Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.

Target information above from: UniProt accession P02462 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1284 Human
  • Entrez Gene: 1285 Human
  • Entrez Gene: 1287 Human
  • Entrez Gene: 1286 Human
  • Entrez Gene: 1282 Human
  • Omim: 120130 Human
  • Omim: 120090 Human
  • Omim: 120070 Human

  • Omim: 303630 Human
  • Omim: 120131 Human
  • SwissProt: P08572 Human
  • SwissProt: Q01955 Human
  • SwissProt: P29400 Human
  • SwissProt: P53420 Human
  • SwissProt: P02462 Human
  • Unigene: 570065 Human
  • Unigene: 591645 Human
  • Unigene: 369089 Human
  • Unigene: 17441 Human

  see all

• Alternative names
  Arresten antibodyCanstatin antibodyCO4A1_HUMAN antibody

  COL4A1 antibodyCOL4A1 NC1 domain antibodyCOL4A2 antibodyCOL4A3 antibodyCOL4A4 antibodyCOL4A5 antibodyCollagen Alpha 1(IV) Chain antibodyCollagen Alpha 2(IV) Chain antibodycollagen alpha-1(IV) chain antibodyCollagen IV Alpha 1 Polypeptide antibodyCollagen IV Alpha 2 Polypeptide antibodyCollagen Of Basement Membrane Alpha 1 Chain antibodyCollagen Of Basement Membrane Alpha 2 Chain antibodyCollagen Type IV Alpha 1 antibodyCollagen Type IV Alpha 2 antibodyCollagen Type IV Alpha 3 antibodyCollagen Type IV Alpha 4 antibodyCollagen Type IV Alpha 5 antibodyDKFZp686I14213 antibodyFLJ22259 antibody

  see all

Anti-Collagen IV antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab21295)
  IHC image of Collagen IV staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21295, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.