Overview
- Product nameAnti-Collagen IV antibodySee all Collagen IV primary antibodies ...
- DescriptionRabbit polyclonal to Collagen IV
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic peptide (Human)
- Positive controlExtracts from HeLa cells.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab52235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/20000. |
| WB | WB: 1/300 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). |
Target
- FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. - Tissue specificityHighly expressed in placenta.
- Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. - Sequence similaritiesBelongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. - DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
- Post-translational
modificationsLysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten. - Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
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Database links
- Entrez Gene: 1284 Human
- Entrez Gene: 1285 Human
- Entrez Gene: 1287 Human
- Entrez Gene: 1286 Human
- Entrez Gene: 1282 Human
- Entrez Gene: 12827 Mouse
- Entrez Gene: 12828 Mouse
- Entrez Gene: 12830 Mouse
- Entrez Gene: 12829 Mouse
- Entrez Gene: 12826 Mouse
- Omim: 120130 Human
- Omim: 120090 Human
- Omim: 120070 Human
- Omim: 303630 Human
- Omim: 120131 Human
- SwissProt: P08572 Human
- SwissProt: Q01955 Human
- SwissProt: P29400 Human
- SwissProt: P53420 Human
- SwissProt: P02462 Human
- SwissProt: P08122 Mouse
- SwissProt: Q9QZS0 Mouse
- SwissProt: Q9QZR9 Mouse
- SwissProt: P02463 Mouse
- Unigene: 570065 Human
- Unigene: 591645 Human
- Unigene: 369089 Human
- Unigene: 17441 Human
- Unigene: 738 Mouse
see all
Target information above from: UniProt accession
P02462
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Arresten antibodyASLN antibodyATS antibody
- CA54 antibodyCanstatin antibodyCO4A1_HUMAN antibodyCOL4A1 antibodyCOL4A1 NC1 domain antibodyCOL4A2 antibodyCOL4A3 antibodyCOL4A4 antibodyCOL4A5 antibodyCollagen Alpha 1(IV) Chain antibodyCollagen Alpha 2(IV) Chain antibodycollagen alpha-1(IV) chain antibodyCollagen IV Alpha 1 Polypeptide antibodyCollagen IV Alpha 2 Polypeptide antibodyCollagen Of Basement Membrane Alpha 1 Chain antibodyCollagen Of Basement Membrane Alpha 2 Chain antibodyCollagen Type IV Alpha 1 antibodyCollagen Type IV Alpha 2 antibodyCollagen Type IV Alpha 3 antibodyCollagen Type IV Alpha 4 antibodyCollagen Type IV Alpha 5 antibodyDKFZp686I14213 antibodyFLJ22259 antibody
see all
Anti-Collagen IV antibody images
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Western blot - Collagen IV antibody (ab52235)All lanes : Anti-Collagen IV antibody (ab52235) at 1/300 dilution
Lane 1 : Extract from HeLa cells, untreated.
Lane 2 : Extract from HeLa cells, treated with the immunising peptide.
Predicted band size : 160 kDa
Observed band size : 160 kDa
References for Anti-Collagen IV antibody (ab52235)
ab52235 has not yet been referenced specifically in any publications.
