Overview

  • Product nameAnti-Collagen V antibodySee all Collagen V primary antibodies ...
  • Description
    Rabbit polyclonal to Collagen V
  • SpecificityNegligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Tested applicationsSandwich ELISA, IHC-Fr, IP, WB, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Collagen V aa 1-1745.

  • Positive control
    • IHC :Dog skeletal muscle tissue, Human pancreas tissue.
  • General notes

    Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582).
IHC-Fr 1/50 - 1/200.
IP 1/5000 - 1/10000.
WB 1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
ICC/IF Use at an assay dependent dilution. PubMed: 18385800
IHC-P 1/500.

Target

  • FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • Involvement in diseaseEhlers-Danlos syndrome 1
    Ehlers-Danlos syndrome 2
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 laminin G-like domain.
  • DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Sulfated on 40% of tyrosines.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P20908 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AB collagen antibody
    • Alpha 1 type V collagen antibody
    • Alpha 2 type V collagen antibody
    • CO5A1_HUMAN antibody
    • Col5A1 antibody
    • Col5A2 antibody
    • COL5A2 protein antibody
    • Col5A3 antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha 2 (V) chain precursor antibody
    • Collagen alpha 2(V) chain antibody
    • Collagen alpha 3(V) chain antibody
    • Collagen alpha-1(V) chain antibody
    • Collagen fetal membrane A polypeptide antibody
    • Collagen type V alpha 1 antibody
    • Collagen type V alpha 2 antibody
    • Collagen type V alpha 3 antibody
    • Collagen V alpha 2 polypeptide antibody
    • CollagenV antibody
    • MGC105115 antibody
    • OTTHUMP00000064637 antibody
    • Pro alpha 1 type V collagen antibody
    • Pro alpha 3(V) collagen antibody
    • Procollagen alpha 2(V) antibody
    • Type V preprocollagen alpha 2 chain antibody
    see all

Anti-Collagen V antibody images

  • ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.

    See Abreview

  • ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.

    See Abreview

  • Anti-Collagen V antibody (ab7046) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 180 kDa
    Observed band size : 180 kDa
    Additional bands at : 122 kDa,54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-Collagen V antibody (ab7046)

This product has been referenced in:
  • Li J  et al. Hyaluronan injection in murine osteoarthritis prevents TGFbeta 1-induced synovial neovascularization and fibrosis and maintains articular cartilage integrity by a CD44-dependent mechanism. Arthritis Res Ther 14:R151 (2012). IHC-P ; Mouse . Read more (PubMed: 22721434) »
  • Schledzewski K  et al. Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors. J Clin Invest : (2011). IHC-P ; Mouse . Read more (PubMed: 21293057) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us with your enquiry.

Ab27478 is a suitable isotype control to use with an unconjugated rabbit polyclonal primary antibody, so I would recommend using it as a negative controlfor ab100810 and ab7046 in IHC. As the IgG...

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Thank you for your inquiry.

For a positive control for ab7046, you can use human pancreas tissue slides as ab4611.

http://www.abcam.com/Pancreas-human-normal-tissue-slides-ab4611.html

For the negative control, you can use ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: .25% · Temperature: 25°C
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Submitted Dec 01 2011

Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (skeletal muscle frozen sections)
Specification skeletal muscle frozen sections
Fixative Acetone
Permeabilization No
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Submitted Sep 20 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (adrenal cortex)
Specification adrenal cortex
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

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Submitted Sep 12 2007

Thank you for your email. I'm afraid that I do not know how neutralizing the pH in your particular assay is going to affect collagen V. To our knowledge this antibody has not been tested in proliferation assays. I'm sorry that I can't be of further ass...

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You want to add glycerol to 50% before freezing. So what your labmate did (vial is 50% antibody, 50% glycerol) is correct.

Thank you for your patience in awaiting a response. Collagen V is over 400kDa.It is important to note that this product will not be supported in reduced conditions. Please use this product in non-reducing conditions. The public-facing data sheet. Thank...

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Sorry, we really don't know if the antibody would detect the type specific collagen fragments.

The immunogen used to generate Rabbit polyclonal to Collagen V was Collagen Type V from adult human and bovine tissues. As the antibody is a polyclonal and was raised agaisnt the whole collagen molecule it will recognise a number of epitopes on the sur...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"