Recombinant Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7889(N)] to Collagen VI alpha 2
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Collagen VI alpha 2 antibody [EPR7889(N)]
See all Collagen VI alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR7889(N)] to Collagen VI alpha 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Collagen VI alpha 2 aa 800-900 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: P12110 -
Positive control
- WB: Human skin lysate IHC-P: Human colon carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7889(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified form, use 1/500 - 1/1000 dilution |
WB |
1/1000 - 1/5000. Predicted molecular weight: 109 kDa.
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Notes |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified form, use 1/500 - 1/1000 dilution |
WB
1/1000 - 1/5000. Predicted molecular weight: 109 kDa. |
Target
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Function
Collagen VI acts as a cell-binding protein. -
Involvement in disease
Defects in COL6A2 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A2 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
Defects in COL6A2 are the cause of myosclerosis autosomal recessive (MYOSAR) [MIM:255600]; also known as myosclerotic myopathy or congenital myosclerosis of Lowenthal. A condition characterized by chronic inflammation of skeletal muscle with hyperplasia of the interstitial connective tissue. The clinical picture includes slender muscles with firm 'woody' consistency and restriction of movement of many joints because of muscle contractures. -
Sequence similarities
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Membrane. Recruited on membranes by CSPG4. - Information by UniProt
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Database links
- Entrez Gene: 1292 Human
- Omim: 120240 Human
- SwissProt: P12110 Human
- Unigene: 420269 Human
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Alternative names
- CO6A2_HUMAN antibody
- COL6A2 antibody
- Collagen alpha 2(VI) chain antibody
see all
Images
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Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855) at 1/1000 dilution (Purified) + Human skin lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 109 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Collagen VI alpha 2 with Purified ab180855 at 1:4000 dilution (0.413 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab180855 has been referenced in 3 publications.
- Chen PY et al. Comprehensive Transcriptomic Analysis and Experimental Validation Identify lncRNA HOXA-AS2/miR-184/COL6A2 as the Critical ceRNA Regulation Involved in Low-Grade Glioma Recurrence. Onco Targets Ther 13:4999-5016 (2020). PubMed: 32581558
- Endicott J et al. Authentication of collagen VI antibodies. BMC Res Notes 10:358 (2017). PubMed: 28755659
- Shaheen NL et al. Extracellular matrix composition modulates angiosarcoma cell attachment and proliferation. Oncoscience 4:178-188 (2017). PubMed: 29344556