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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Anti-Collagen X antibody
See all Collagen X products (3) ...
Description
Rabbit polyclonal to Collagen X
Specificity
ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
Tested applications
IHC-P, ICC/IF, WB, IP, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Cow, Human
Immunogen
Full length native protein (purified) Type X collagen from rat chondrosarcoma cells
Properties
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Western blot - Collagen X antibody (ab58632)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.(We recommend overnight incubation with the antibody. For antigen retrieval, we suggest digestion with 0.25% trypsin in 1mM EDTA for 3 minutes at 37C. )
ICC/IF: Use at an assay dependent concentration.
WB: 1/100 - 1/300.
IP
IP: 1/20 - 1/40.IP: 1/20 - 1/40.
IHC-Fr: Use at an assay dependent dilution. (Enzymatic or heat (41°C) pretreatment is recommended.)
Target
Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
Sequence similarities
Contains 1 C1q domain.
Post-translational
modifications
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ03692
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Database links
- Entrez Gene: 282416 Cow
- Entrez Gene: 1300 Human
- Entrez Gene: 12813 Mouse
- Entrez Gene: 25681 Rat
- Omim: 120110 Human
- SwissProt: P23206 Cow
- SwissProt: A1L4P2 Human
- SwissProt: Q03692 Human
see all
Anti-Collagen X antibody images:
Western blot - Collagen X antibody (ab58632)
Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 66 + 64 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,50 kDa,98 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 150 seconds
The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.
References for Anti-Collagen X antibody (ab58632)
This product has been referenced in:
- Mengatto CMet al. Circadian rhythm and cartilage extracellular matrix genes in osseointegration: a genome-wide screening of implant failure by vitamin d deficiency. PLoS One 6:e15848 (2011). ICC/IF; Mouse.Read more (PubMed: 21264318) »
- Eliyahu Eet al. Anti-TNF-Alpha Therapy Enhances the Effects of Enzyme Replacement Therapy in Rats with Mucopolysaccharidosis Type VI. PLoS One 6:e22447 (2011). WB; Rat.Read more (PubMed: 21887218) »
See all 6 publications for this product
Publishing research using ab58632? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"