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Full length native protein (purified) Type X collagen from rat chondrosarcoma cells
Our Abpromise guarantee covers the use of ab58632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend overnight incubation with the antibody. For antigen retrieval, we suggest digestion with 0.25% trypsin in 1mM EDTA for 3 minutes at 37C.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/100 - 1/300.|
|IP||1/20 - 1/40.|
|IHC-Fr||Use at an assay dependent concentration. Enzymatic or heat (41°C) pretreatment is recommended.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"