Complement C4 Human ELISA Kit (ab108824)
- Product nameComplement C4 Human ELISA KitSee all Complement C4 kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.9%
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.3 µg/ml
- Range0.313 µg/ml - 5 µg/ml
- Assay time3h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Complement C4 Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C4 levels in plasma and serum.
A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement C4 is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C4 captured in plate.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human Complement C4 (Lyophilized) 1 vial Chromogen Substrate 1 x 8ml Complement C4 Microplate (12 x 8 well strips) 1 unit Complement C4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
- Research Areas
- Acidic complement C4
- Basic complement C4
- C3 and PZP like alpha 2 macroglobulin domain containing protein 2
- C3 and PZP like alpha 2 macroglobulin domain containing protein 3
- Complement C4 A
- Complement C4 B
- Complement component 4A
- Complement component 4B
Our Abpromise guarantee covers the use of ab108824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Complement C4 Human ELISA Kit (ab108824)
ab108824 has not yet been referenced specifically in any publications.