Complement C4 Human ELISA Kit (ab108824)
Overview
- Product nameComplement C4 Human ELISA KitSee all Complement C4 kits ...
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeSandwich
- Sensitivity= 0.3 µg/ml
- Range0.313 µg/ml - 5 µg/ml
- Recovery
103 %
- Assay time3h 00m
- Species reactivityReacts with: Human
- Product overview
Complement protein C4 is the second component to react in the complement sequence. It is a beta-globulin with a sedimentation coefficient of 18.7 and a molecular weight of 240,000. The C4 component participates in the initial step of activation of classical complement pathway. Lower levels of complement C4 in serum was associated with primary biliary cirrhosis, human systemic lupus erythematosus, and chronic liver disease.
ab108824 Complement C4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human complement C4 in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human complement C4 in less than 3 hours. A polyclonal antibody specific for human complement C4 has been pre-coated onto a 96-well microplate with removable strips. Complement C4 in standards and samples is competed by a biotinylated complement C4 sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated Complement C4 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Complement C4 Microplate 1 unit Human Complement C4 Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml - Research Areas
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Alternative names
- Acidic complement C4Basic complement C4C3 and PZP like alpha 2 macroglobulin domain containing protein 2
- C3 and PZP like alpha 2 macroglobulin domain containing protein 3C4BCO4Complement C4 AComplement C4 BComplement component 4AComplement component 4BCPAMD2CPAMD3
see all
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
Applications
Our Abpromise guarantee covers the use of ab108824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Complement C4 Human ELISA Kit images
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ELISA - Complement C4 Human ELISA Kit (ab108824)Human Complement C4 Standard Curve
Protocols
References for Complement C4 Human ELISA Kit (ab108824)
ab108824 has not yet been referenced specifically in any publications.
