Complement C4 Human ELISA Kit (ab108825)
- Product nameComplement C4 Human ELISA KitSee all Complement C4 kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.6%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine
- Assay typeSandwich (quantitative)
- Sensitivity= 0.08 ng/ml
- Range0.078 ng/ml - 20 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Complement C4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C4 levels in urine, milk, saliva and cell culture supernatants.
A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C4 captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Biotinylated Human Complement C4 Antibody 1 x 80µl 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 8ml Complement C4 Microplate (12 x 8 well strips) 1 unit Complement C4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
- Research Areas
- Acidic complement C4
- Basic complement C4
- C3 and PZP like alpha 2 macroglobulin domain containing protein 2
- C3 and PZP like alpha 2 macroglobulin domain containing protein 3
- Complement C4 A
- Complement C4 B
- Complement component 4A
- Complement component 4B
Our Abpromise guarantee covers the use of ab108825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Complement C4 Human ELISA Kit (ab108825)
ab108825 has not yet been referenced specifically in any publications.