Complement C5 Human ELISA Kit (ab125963)
- Product nameComplement C5 Human ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 4.3%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Serum, Plasma
- Assay typeCompetitive
- Sensitivity0.3 ng/ml
- Range0.313 ng/ml - 20 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Human Complement Component 5 (C5) is the fifth component of the complement system. C5 has a molecular weight of about 195 kDa, and matures into a heterodimers with a- and ß-chains of 120 kDa and 75 kDa, respectively. Upon complement activation, C5 is cleaved into a small C5a and larger C5b polypeptides by C5 convertase. The potent pro-inflammatory anaphylatoxin C5a binds to receptors C5aR and C5L2 to initiate acute inflammatory responses. The C5a-mediated early pro-inflammatory responses include sepsis, systemic lupus erythamatosis and cerebral malaria. The larger C5b interact with complement components C6, C7, C8, and C9 to form a membrane attack complex C5b-9 which is involved in cell apoptosis, cell activation and production of proinflammatory mediators. C5 deficiency is associated with a severe infantile dermatitis Leiner's disease.
ab125963 Complement C5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C5 in Human plasma, serum, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C5 in less than 4 hours. A polyclonal antibody specific for C5 has been pre-coated onto a microplate. C5 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C5, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human Complement C5 Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Complement C5 Microplate 1 x 96 tests Human Complement C5 Standard (lyophilized) 1 vial Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 8µl Wash Buffer Concentrate (20x) 2 x 30ml
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
Contains 1 NTR domain.
- anaphylatoxin C5a analogC3 and PZP-like alpha-2-macroglobulin domain-containing protein 4C5
- CO5_HUMANcomplement C5Complement C5 alpha'' chainComplement component 5CPAMD4
Our Abpromise guarantee covers the use of ab125963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Complement C5 Human ELISA Kit (ab125963)
ab125963 has not yet been referenced specifically in any publications.