Overview

  • Product nameComplement C5 Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Overall 4.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.3 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Complement C5 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C5 in Human plasma, serum, saliva, milk, CSF and cell culture supernatants.

    A Complement C5 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C5 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C5 captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionActivation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in diseaseDefects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • anaphylatoxin C5a analog
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
    • C5
    • CO5_HUMAN
    • complement C5
    • Complement C5 alpha'' chain
    • Complement component 5
    • CPAMD4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab125963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Complement C5 Human ELISA Kit images

  • Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab125963

Protocols

References for Complement C5 Human ELISA Kit (ab125963)

ab125963 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab125963.


I have confirmed that this kit will react withboth C5 and C5a.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Thank you for contacting us.

The kit standard is purified from human plasma with >95% purity by SDS-PAGE. The MW is 190,000 Da (2 chains).

I hope this information is helpful to you. Please do not hesitate to contact us if you ne...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"