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Overview

  • Product nameComplement C5 Human ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    0.3 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Complement Component 5 (C5) is the fifth component of the complement system. C5 has a molecular weight of about 195 kDa, and matures into a heterodimers with a- and ß-chains of 120 kDa and 75 kDa, respectively. Upon complement activation, C5 is cleaved into a small C5a and larger C5b polypeptides by C5 convertase. The potent pro-inflammatory anaphylatoxin C5a binds to receptors C5aR and C5L2 to initiate acute inflammatory responses. The C5a-mediated early pro-inflammatory responses include sepsis, systemic lupus erythamatosis and cerebral malaria. The larger C5b interact with complement components C6, C7, C8, and C9 to form a membrane attack complex C5b-9 which is involved in cell apoptosis, cell activation and production of proinflammatory mediators. C5 deficiency is associated with a severe infantile dermatitis Leiner's disease.

    ab125963 Complement C5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C5 in Human plasma, serum, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C5 in less than 4 hours. A polyclonal antibody specific for C5 has been pre-coated onto a microplate. C5 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C5, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • FunctionActivation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in diseaseDefects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01031 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        anaphylatoxin C5a analogC3 and PZP-like alpha-2-macroglobulin domain-containing protein 4C5
        CO5_HUMANcomplement C5Complement C5 alpha'' chainComplement component 5CPAMD4
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab125963 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Complement C5 Human ELISA Kit images

      • ELISA - Complement C5 Human ELISA Kit (ab125963)
        ELISA - Complement C5 Human ELISA Kit (ab125963)
        Standard curve

      Protocols

      References for Complement C5 Human ELISA Kit (ab125963)

      ab125963 has not yet been referenced specifically in any publications.

      Publishing research using ab125963 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab125963 Submit a Question for ab125963

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      Votes: Highest First

      I have a question in reference to your C5 kit (ab125963). Specifically, I am looking for an assay that detects only unactivated C5, not C5a or C5a-des-Arg. I was not clear from your product information if your ELISA measures both C5 and C5a or just the un...

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      Thank you for contacting Abcam regarding ab125963.


      I have confirmed that this kit will react withboth C5 and C5a.


      I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

      Is the protein used as standard from a native source or rather recombinant?
      What is the MW of the protein standard?

      Thank you for contacting us.

      The kit standard is purified from human plasma with >95% purity by SDS-PAGE. The MW is 190,000 Da (2 chains).

      I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"