Complement C6 Human ELISA Kit (ab125965)
- Product nameComplement C6 Human ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 4.1%
- Tests1 x 96 well plate
- Sample typeSaliva, Milk, Urine, Serum, Plasma, Cell culture extracts
- Assay typeCompetitive
- Sensitivity0.7 ng/ml
- Range0.78 ng/ml - 50 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Human Complement Component 6 (C6) is a single-chain glycoprotein consisting of 913 amino acid residues with a molecular mass of about 102 kDa. C6 is a part of the lytic membrane attack complex during complement activation. Cleavage of C5 into C5a and C5b by C5 convertase triggers binding of plasma C6 to C5b. Once the C5b-6 complex forms, C7, C8, and C9 add sequentially to create a transmembrane channel structure. Complete deficiency of C6 (C6Q0) leads to an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease. In animal models, genetic C6 deficiency accelerates axonal regeneration and reduces atherosclerosis.
ab125965 Complement C6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C6 in Human plasma, serum, saliva, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C6 in less than 4 hours. A polyclonal antibody specific for C6 has been pre-coated onto a microplate. C6 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C6, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human Complement C6 Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Complement C6 Microplate 1 x 96 tests Human Complement C6 Standard (lyophilized) 1 vial Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 8µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 3 TSP type-1 domains.
modificationsAll cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module.
- C6CO6_HUMANComplement component C6
Our Abpromise guarantee covers the use of ab125965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Complement C6 Human ELISA Kit (ab125965)
ab125965 has not yet been referenced specifically in any publications.