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Overview

  • Product nameComplement C6 Human ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts
  • Assay typeCompetitive
  • Sensitivity
    0.7 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Complement Component 6 (C6) is a single-chain glycoprotein consisting of 913 amino acid residues with a molecular mass of about 102 kDa. C6 is a part of the lytic membrane attack complex during complement activation. Cleavage of C5 into C5a and C5b by C5 convertase triggers binding of plasma C6 to C5b. Once the C5b-6 complex forms, C7, C8, and C9 add sequentially to create a transmembrane channel structure. Complete deficiency of C6 (C6Q0) leads to an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease. In animal models, genetic C6 deficiency accelerates axonal regeneration and reduces atherosclerosis.

    ab125965 Complement C6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C6 in Human plasma, serum, saliva, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C6 in less than 4 hours. A polyclonal antibody specific for C6 has been pre-coated onto a microplate. C6 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C6, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • FunctionConstituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
  • Involvement in diseaseDefects in C6 are the cause of complement component 6 deficiency (C6D) [MIM:612446]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 2 Kazal-like domains.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 2 Sushi (CCP/SCR) domains.
    Contains 3 TSP type-1 domains.
  • Post-translational
    modifications    All cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P13671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        C6CO6_HUMANComplement component C6
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab125965 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Complement C6 Human ELISA Kit images

      • ELISA - Complement C6 Human ELISA Kit (ab125965)
        ELISA - Complement C6 Human ELISA Kit (ab125965)
        Standard curve

      Protocols

      References for Complement C6 Human ELISA Kit (ab125965)

      ab125965 has not yet been referenced specifically in any publications.

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