Complement C7 Human ELISA Kit (ab125964)
- Product nameComplement C7 Human ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 4.3%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeCompetitive
- Sensitivity0.6 ng/ml
- Range0.625 ng/ml - 40 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Human Complement Component 7 (C7) C7 is a single-chain glycoprotein consisting of 821 amino acid residues with a molecular weight of about 97 kDa. The amino-terminal of C7 has 23-30% homology with Complement Components C8 and C9. C7 is one of five complement proteins C5b, C6, C7, C8, and C9 that assemble on pathogen membranes to form the lethal membrane attack complex. It serves as a membrane anchor for the C5b-7 complex. C7 deficiency exhibits an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease. Plasma C7 is also associated with asthma exacerbation, age-related macular degeneration, and multiple sclerosis.
ab125964 Complement C7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C7 in human plasma, serum, urine, milk, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C7 in 4 hours. A polyclonal antibody specific for C7 has been pre-coated onto a microplate. C7 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C7, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human Complement C7 Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Complement C7 Microplate 1 x 96 tests Human Complement C7 Standard (lyophilized) 1 vial Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 2 TSP type-1 domains.
modificationsC7 has 28 disulfide bridges.
- C7CO7_HUMANComplement component 7Complement component C7
Our Abpromise guarantee covers the use of ab125964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Complement C7 Human ELISA Kit (ab125964)
ab125964 has not yet been referenced specifically in any publications.