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Overview

  • Product nameComplement C7 Human ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    0.6 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Complement Component 7 (C7) C7 is a single-chain glycoprotein consisting of 821 amino acid residues with a molecular weight of about 97 kDa. The amino-terminal of C7 has 23-30% homology with Complement Components C8 and C9. C7 is one of five complement proteins C5b, C6, C7, C8, and C9 that assemble on pathogen membranes to form the lethal membrane attack complex. It serves as a membrane anchor for the C5b-7 complex. C7 deficiency exhibits an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease. Plasma C7 is also associated with asthma exacerbation, age-related macular degeneration, and multiple sclerosis.

    ab125964 Complement C7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C7 in human plasma, serum, urine, milk, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C7 in 4 hours. A polyclonal antibody specific for C7 has been pre-coated onto a microplate. C7 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C7, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • FunctionConstituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
  • Involvement in diseaseDefects in C7 are a cause of complement component 7 deficiency (C7D) [MIM:610102]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 2 Sushi (CCP/SCR) domains.
    Contains 2 TSP type-1 domains.
  • Post-translational
    modifications    C7 has 28 disulfide bridges.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P10643 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        C7CO7_HUMANComplement component 7Complement component C7
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab125964 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Complement C7 Human ELISA Kit images

      • ELISA - (ab125964)
        ELISA - (ab125964)
        Standard curve

      Protocols

      References for Complement C7 Human ELISA Kit (ab125964)

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