MitoSciences (MS131)

Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)

Overview

  • Product nameComplex I Human Protein Quantity Dipstick Assay Kit
    See all Complex I kits
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeSandwich (quantitative)
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    ab109722 contains 48 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex in human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.


    Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the Complex I enzyme complex. One antibody is immobilized on the nitrocellulose membrane in a thin line perpendicular to the length of the dipstick, while the other is gold-conjugated and combined with the sample mix. The sample contents containing the gold-conjugated mAb wick past the mAb immobilized on the dipstick. When assembled Complex I is present in the sample, a red line appears at the site of the anti-Complex I antibody line. The signal intensity is directly related to the amount of Complex I in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. To identify defects in Complex I that do not affect enzyme assembly combine this assay with ab109720 to determine the relative specific activity of Complex I.

  • Notes

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C.

    For long-term storage, all buffers can be stored at 4°C.

  • Tested applicationsSandwich ELISAmore details
  • PlatformReagents

Properties

  • Alternative names
    • NADH dehydrogenase

Applications

Our Abpromise guarantee covers the use of ab109722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Complex I Human Protein Quantity Dipstick Assay Kit images

  • Figure 1. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.
  • Figure 2. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.
  • Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

Protocols

References for Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)

This product has been referenced in:
  • Machado AM  et al. Helicobacter pylori infection affects mitochondrial function and DNA repair, thus, mediating genetic instability in gastric cells. Mech Ageing Dev N/A:N/A (2013). Read more (PubMed: 24012633) »
  • Ceusters JD  et al. Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts. Res Vet Sci 95:870-8 (2013). Functional Studies ; Horse . Read more (PubMed: 24099743) »

See all 8 Publications for this product

Product Wall



We don’t have a lot of experience with blood samples and Dipstick assays. From the limited experience we have, I suggest to always run on fresh EDTA blood (store at 4C for no more than 1 week). If this is not possible, I suggest to free...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"