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Product name
Complex I Immunocapture Kit
See all Complex I products (7) ...
Assay type
Quantitative
Product overview
250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I from heart mitochondria.
ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857/MS910).
Tested applications
Properties
Storage instructions
Store at +4°C.
| Components | 250 µg | 500 µg | 750 µg |
|---|---|---|---|
| monoclonal antibody irreversibly cross-linked to protein G-agarose beads | 1 x 250µg | 1 x 500µg | 1 x 750µg |
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Immunoprecipitation - Complex I Immunocapture Kit (ab109711)
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Applications
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Our Abpromise guarantee covers the use of ab109711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Complex I Immunocapture Kit images:
Immunoprecipitation - Complex I Immunocapture Kit (ab109711)
Complex I immunoprecipitation using antibody ab109798/MS101c crosslinked to protein G-agarose beads as product ab109711
Protocols
References for Complex I Immunocapture Kit (ab109711)
This product has been referenced in:
- Guy J et al. Efficiency and safety of AAV-mediated gene delivery of the human ND4 complex I subunit in the mouse visual system. Invest Ophthalmol Vis Sci 50:4205-14 (2009).Read more (PubMed: 19387075) »
- Chinta SJ et al. Reactive oxygen species regulation by AIF- and complex I-depleted brain mitochondria. Free Radic Biol Med 46:939-47 (2009).Read more (PubMed: 19280713) »
See all 13 publications for this product
Publishing research using ab109711? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"