![Immunoprecipitation - Complex I Immunocapture antibody [18G12BC2] (ab109798) image](#Complex-I-Immunocapture-Primary-antibodies-ab109798-2.jpg)
![Immunocytochemistry - Complex I Immunocapture antibody [18G12BC2] (ab109798) image](#Complex-I-Immunocapture-Primary-antibodies-ab109798-3.jpg)
![- Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798) image](#Complex-I-Immunocapture-Primary-antibodies-ab109798-5.jpg)
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Product name
Anti-Complex I Immunocapture antibody [18G12BC2]
Description
Mouse monoclonal [18G12BC2] to Complex I Immunocapture
Tested applications
IP, ICC, In-Cell ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Positive control
fibroblasts, HL-60 cells, tissue mitochondria
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
18G12BC2
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 100-1 µg/mg of lysate. 100 µg mAb can capture at least 25 µg complex I from 1 mg solubilized bovine heart mitochondria.
ICC: Use at an assay dependent dilution.
In-Cell ELISA: Use a concentration of 1 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml.
Target
Relevance
Complex I, or NADH ubiquinone oxidoreductase, is a large protein complex of 950,000 Da molecular weight made up by 45 to 46 different subunits. A total of seven of the subunits of the complex are encoded by mitochondrial DNA, while the remainder subunits are nuclear encoded, which are translated in the cytosol and translocated into the organelle for assembly at the inner membrane. The enzyme complex catalyses electron entry from NADH via a flavin (FMN) and several non-heme iron centers. Complex I is sensitive to a wide range of inhibitors, many of which are pesticides or other common environmental toxins, such as rotenone. Complex I dysfunction is a common cause of genetic OXPHOS defects. Altered functioning of this complex is also thought to contribute to several neurological disorders including Parkinson’s disease and schizophrenia. Also, there is evidence of Complex I involvement in diabetes.
Anti-Complex I Immunocapture antibody [18G12BC2] images:
Immunoprecipitation - Complex I Immunocapture antibody [18G12BC2] (ab109798)
![Immunoprecipitation - Complex I Immunocapture antibody [18G12BC2] (ab109798)](/ps/datasheet/images/109/ab109798/Complex-I-Immunocapture-Primary-antibodies-ab109798-2.jpg)
Complex I immunoprecipitation for antibody ab109798 crosslinked to protein G-agarose beads as product ab109711.
Immunocytochemistry - Complex I Immunocapture antibody [18G12BC2] (ab109798)
![Immunocytochemistry - Complex I Immunocapture antibody [18G12BC2] (ab109798)](/ps/datasheet/images/109/ab109798/Complex-I-Immunocapture-Primary-antibodies-ab109798-3.jpg)
Immunocytochemistry image of ab109798 stained fibroblasts cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab109798, 1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 4884 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
- Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)
![- Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)](/ps/datasheet/images/109/ab109798/Complex-I-Immunocapture-Primary-antibodies-ab109798-5.jpg)
HL-60 cells were stained with 1 µg/mL Complex I antibody ab109798 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
References for Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)
This product has been referenced in:
- Lund KC & Wallace KB Adenosine 3',5'-cyclic monophosphate (cAMP)-dependent phosphoregulation of mitochondrial complex I is inhibited by nucleoside reverse transcriptase inhibitors. Toxicol Appl Pharmacol 226:94-106 (2008).Read more (PubMed: 17904600) »
- Doughan AK & Dikalov SI Mitochondrial redox cycling of mitoquinone leads to superoxide production and cellular apoptosis. Antioxid Redox Signal 9:1825-36 (2007).Read more (PubMed: 17854275) »
See all 9 publications for this product
Publishing research using ab109798? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"