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Complex II Enzyme Activity Microplate Assay Kit is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Succinate, Ubiquinone 2, DCPIP and Lipid?Phospholipd Mix should be stored at -80°C. All other components should be stored at 4°C.
|10X Blocking Solution||1 x 5ml|
|10X Detergent||2 x 1ml|
|20X Buffer||1 x 15ml|
|Complex II Activity Buffer||1 x 25ml|
|DCIP||1 x 250µl|
|Phospholipids||1 x 6ml|
|Pre-coated 96-well microplate (12 strips)||1 unit|
|Succinate||1 x 500µl|
|Ubiquinone 2||1 x 60µl|
Our Abpromise guarantee covers the use of ab109908 in the following tested applications.
|Functional Studies||Use at an assay dependent dilution.|