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Complex II Enzyme Activity Microplate Assay Kit (ab109908)

MSCatalog No. MS241

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Overview

Product name

Complex II Enzyme Activity Microplate Assay Kit
See all Complex II products (5) ...

Assay type

Enzyme activity

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Product overview

Complex II Enzyme Activity Microplate Assay Kit is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.

Notes

Succinate, Ubiquinone 2 and DCPIP should be stored at -80°C. Lipid Mix should be stored at -20°C. All other components should be stored at 4°C.

Tested applications

Functional Studiesmore details

Properties

Storage instructions

Please see Notes section

Components 96 tests
10X Blocking Solution1 x 5ml
10X Detergent2 x 1ml
20X Buffer1 x 15ml
Complex II Activity Buffer1 x 25ml
DCPIP1 x 250µl
Lipid Mix (Phospholipids)1 x 6ml
Pre-coated 96-well microplate (12 strips)1 unit
Succinate1 x 500µl
Ubiquinone 21 x 60µl
  • Functional Studies - Complex II Enzyme Activity Microplate Assay Kit (ab109908)Functional Studies - Complex II Enzyme Activity Microplate Assay Kit (ab109908) image (enlarge)

  • - Complex II Enzyme Activity Microplate Assay Kit (ab109908)- Complex II Enzyme Activity Microplate Assay Kit (ab109908) image (enlarge)

  • - Complex II Enzyme Activity Microplate Assay Kit (ab109908)- Complex II Enzyme Activity Microplate Assay Kit (ab109908) image (enlarge)

Relevance

Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.

Alternative names

  • MS1036
  • MS241
  • MTOX2
  • Succinate coenzyme Q reductase

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    Functional Studies

     FuncS

    FuncS

Complex II Enzyme Activity Microplate Assay Kit images:

  Functional Studies - Complex II Enzyme Activity Microplate Assay Kit (ab109908)

Functional Studies - Complex II Enzyme Activity Microplate Assay Kit (ab109908)

Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, substrate is added, and enzyme activity is analyzed by measuring the change in absorbance of either the substrate or the product of the reaction (depending upon which enzyme is being analyzed). By analyzing the enzyme's activity in an isolated context, outside of the cell and free from any other variables, an accurate measurement of the enzyme's functional state can be understood.

  - Complex II Enzyme Activity Microplate Assay Kit (ab109908)

- Complex II Enzyme Activity Microplate Assay Kit (ab109908)

Figure 1. Example of raw data. Note the lag period before activity. Also note the activity of mitochondria (BHM, bovine heart mitochondria) is higher than whole cell lysate (HepG2, human hepatoblastoma) and the reaction ends at >1600 seconds because the substrates are used up.

  - Complex II Enzyme Activity Microplate Assay Kit (ab109908)

- Complex II Enzyme Activity Microplate Assay Kit (ab109908)

Figure 2. This assay is compatible with different sample types such as mitochondria, tissue or cell lysates and in multiple species including human and rodent samples. Typical linear range data are shown for ab109908.

References for Complex II Enzyme Activity Microplate Assay Kit (ab109908)

This product has been referenced in:

  • Li FC  et al. Bioenergetics failure and oxidative stress in brain stem mediates cardiovascular collapse associated with fatal methamphetamine intoxication. PLoS One 7:e30589 (2012). Rat.Read more (PubMed: 22276218) »
  • Ichikawa Y  et al. Disruption of ATP-binding cassette B8 in mice leads to cardiomyopathy through a decrease in mitochondrial iron export. Proc Natl Acad Sci U S A 109:4152-7 (2012).Read more (PubMed: 22375032) »

See all 3 publications for this product

Publishing research using ab109908? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"