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MitoSciences (MS201)

Complex II Immunocapture Kit (ab109799)

Q&A (1)Specific References (5)

Overview

  • Product nameComplex II Immunocapture KitSee all Complex II kits ...
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.

    The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIP more details

Properties

  • RelevanceComplex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
    • Alternative names
        SDHSuccinate coenzyme Q reductase Succinate dehydrogenase
  • Database links
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    • Applications

    Our Abpromise guarantee covers the use of ab109799 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IP IP

    Complex II Immunocapture Kit images

    • Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
      Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
      Complex II immunoprecipitation using antibody ab109865 /MS101c crosslinked to protein G-agarose beads as product ab109799

    Protocols

    References for Complex II Immunocapture Kit (ab109799)

    This product has been referenced in:
    • Garcia-Diaz B  et al. Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation. Am J Hum Genet 91:729-36 (2012). Read more (PubMed: 23022099) »
    • Finley LW  et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP ; Mouse . Read more (PubMed: 21858060) »

    See all 5 Publications for this product

    Publishing research using ab109799 ? Please let us know so that we can cite the reference in this datasheet

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    The protocol says to use at least 5 uL per sample- how many uL are provided per 250 ug vial? How many samples can be run with the 250 ug kit?

    Thanks for your call today and for your interest in our complex II immunocapture kit.

    I've heard back from the lab, and the 250 ug kit will come as as 25 uL solution.

    I hope this information is useful, but if there is anything else tha...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"