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Read our guarantee »Overview
Product name
Complex II Immunocapture Kit
See all Complex II products (5) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.
The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Tested applications
Properties
Storage instructions
Store at +4°C.
| Components | 250 µg | 500 µg | 750 µg |
|---|---|---|---|
| monoclonal Complex II antibody irreversibly cross-linked to protein G-agarose beads | 1 x 250µg | 1 x 500µg | 1 x 750µg |
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Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
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Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
Alternative names
- MS1036
- MS241
- MTOX2
- Succinate coenzyme Q reductase
Database links
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab109799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Complex II Immunocapture Kit images:
Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
Complex II immunoprecipitation using antibody ab109865 /MS101c crosslinked to protein G-agarose beads as product ab109799
Protocols
References for Complex II Immunocapture Kit (ab109799)
This product has been referenced in:
- Finley LW et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP; Mouse.Read more (PubMed: 21858060) »
- Paddenberg R et al. Impact of modulators of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) on hypoxic pulmonary vasoconstriction. Adv Exp Med Biol 648:361-8 (2009).Read more (PubMed: 19536500) »
See all 4 publications for this product
Publishing research using ab109799? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"