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Complex II Immunocapture Kit (ab109799)

MSCatalog No. MS201-250

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Overview

Product name

Complex II Immunocapture Kit
See all Complex II products (5) ...

Assay type

Quantitative

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Product overview

250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.

The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.

Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

Tested applications

IPmore details

Properties

Storage instructions

Store at +4°C.

Components 250 µg 500 µg 750 µg
monoclonal Complex II antibody irreversibly cross-linked to protein G-agarose beads1 x 250µg1 x 500µg1 x 750µg
  • Immunoprecipitation - Complex II Immunocapture Kit (ab109799)Immunoprecipitation - Complex II Immunocapture Kit (ab109799) image (enlarge)

Relevance

Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.

Alternative names

  • MS1036
  • MS241
  • MTOX2
  • Succinate coenzyme Q reductase

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Complex II Immunocapture Kit images:

  Immunoprecipitation - Complex II Immunocapture Kit (ab109799)

Immunoprecipitation - Complex II Immunocapture Kit (ab109799)

Complex II immunoprecipitation using antibody ab109865 /MS101c crosslinked to protein G-agarose beads as product ab109799

References for Complex II Immunocapture Kit (ab109799)

This product has been referenced in:

  • Finley LW  et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP; Mouse.Read more (PubMed: 21858060) »
  • Paddenberg R  et al. Impact of modulators of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) on hypoxic pulmonary vasoconstriction. Adv Exp Med Biol 648:361-8 (2009).Read more (PubMed: 19536500) »

See all 4 publications for this product

Publishing research using ab109799? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"