Complex II Immunocapture Kit (ab109799)
- Product nameComplex II Immunocapture KitSee all Complex II kits ...
- Assay typeQuantitative
- Species reactivityReacts with: Mouse, Rat, Cow, Human
- Product overview
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.
The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
- Tested applicationsIP more details
Components 250 µg 500 µg 750 µg monoclonal Complex II antibody irreversibly cross-linked to protein G-agarose beads 1 x 250µg 1 x 500µg 1 x 750µg
- Research Areas
- SDHSuccinate coenzyme Q reductase Succinate dehydrogenase
Our Abpromise guarantee covers the use of ab109799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Complex II Immunocapture Kit images
Complex II immunoprecipitation using antibody ab109865 /MS101c crosslinked to protein G-agarose beads as product ab109799
References for Complex II Immunocapture Kit (ab109799)
This product has been referenced in:
- Garcia-Diaz B et al. Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation. Am J Hum Genet 91:729-36 (2012). Read more (PubMed: 23022099) »
- Finley LW et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP ; Mouse . Read more (PubMed: 21858060) »