MitoSciences (MS201c)

Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

Overview

  • Product nameAnti-Complex II Immunocapture antibody [4H12BG12AG2]
  • Description
    Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture
  • SpecificityHuman, bovine, mouse
  • Tested applicationsICC/IF, WB, IP more details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Bovine Heart Complex II, with traces of Complex III.

  • Positive control
    • Bovine heart and Human mitochondria. IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab109865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. PubMed: 21346097
IP Use at 100-1 µg/mg of lysate.

Target

  • RelevanceComplex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
  • Cellular localizationMitochondrial

Anti-Complex II Immunocapture antibody [4H12BG12AG2] images

  • ICC/IF image of ab109865 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109865, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Staining of Complex II Lane 1: 0.1 mg Bovine mitochondrial lysate Lane 2: 0.5 mg Human mitochondrial lysate Immunoprecipitated using ab109865 at 100 µg/mg lysate

References for Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

This product has been referenced in:

See all 7 Publications for this product

Product Wall

Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"