Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)


  • Product name
    Anti-Complex II Immunocapture antibody [4H12BG12AG2]
  • Description
    Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture
  • Specificity
    Human, bovine, mouse
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Bovine Heart Complex II, with traces of Complex III.

  • Positive control
    • Bovine heart and Human mitochondria. IF/ICC: HeLa cell line
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab109865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. PubMed: 21346097
IP Use at 100-1 µg/mg of lysate.


  • Relevance
    Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
  • Cellular localization

Anti-Complex II Immunocapture antibody [4H12BG12AG2] images

  • ICC/IF image of ab109865 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109865, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Staining of Complex II Lane 1: 0.1 mg Bovine mitochondrial lysate Lane 2: 0.5 mg Human mitochondrial lysate Immunoprecipitated using ab109865 at 100 µg/mg lysate

References for Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

This product has been referenced in:
  • Sakakibara I  et al. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skelet Muscle 6:30 (2016). WB ; Mouse . Read more (PubMed: 27597886) »
  • Jonckheere AI  et al. A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy. Brain 136:1544-54 (2013). Read more (PubMed: 23599390) »

See all 8 Publications for this product

Product Wall

This antibody has been used in western blot in the following publication:

We do fully guarantee the antibody to work in WB however, we feel that a better option for ...

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Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I Complex IV http://www.mi...

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