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MitoSciences (MS202)

Complex II WB Antibody Cocktail (ab110410)

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Overview

  • Product nameComplex II WB Antibody CocktailSee all Complex II kits ...
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).

    Cocktail Antibodies:

    Mouse C-V-alpha [15H4C4] monoclonal:

    Amount: 50 µg

    Working concentration: 1 µg/ml

    Mouse C-II-30 (FeS) [21A11AE7] monoclonal:

    Amount: 250 µg

    Working concentration: 5 µg/ml

    Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:

    Amount: 5 µg

    Working concentration: 0.1 µg/ml

  • Tested applicationsWB more details

Properties

  • RelevanceComplex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
    • Alternative names
        MS202-300SDHSuccinate coenzyme Q reductase Succinate dehydrogenase
  • Database links
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    • Applications

    Our Abpromise guarantee covers the use of ab110410 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.

    Complex II WB Antibody Cocktail images

    • Western blot - Complex II WB Antibody Cocktail (ab110410)
      Western blot - Complex II WB Antibody Cocktail (ab110410)
      All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution

      Lane 1 : human heart mitochondria at 10 µg
      Lane 2 : Immunoprecipitated Complex II at 0.5 µg

    References for Complex II WB Antibody Cocktail (ab110410)

    This product has been referenced in:
    • Guerra F  et al. Placing mitochondrial DNA mutations within the progression model of type I endometrial carcinoma. Hum Mol Genet 20:2394-405 (2011). WB ; Human . Read more (PubMed: 21470976) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"