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Product name
Complex II WB Antibody Cocktail
See all Complex II products (5) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).
Cocktail Antibodies:
Mouse C-V-alpha [15H4C4] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse C-II-30 (FeS) [21A11AE7] monoclonal:
Amount: 250 µg
Working concentration: 5 µg/ml
Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:
Amount: 5 µg
Working concentration: 0.1 µg/ml
Tested applications
Properties
Storage instructions
Store at +4°C. Do not freeze.
| Components | 305 µg | 610 µg |
|---|---|---|
| Pre-mixed solution of 3 monoclonal antibodies | 1 x 305µg | 1 x 610µg |
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Western blot - Complex II WB Antibody Cocktail (ab110410)
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Relevance
Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
Alternative names
- MS1036
- MS202-300
- MS241
see all
Database links
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.
Complex II WB Antibody Cocktail images:
Western blot - Complex II WB Antibody Cocktail (ab110410)
All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution
Lane 1 : human heart mitochondria at 10 µg
Lane 2 : Immunoprecipitated Complex II at 0.5 µg
The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa)and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).
References for Complex II WB Antibody Cocktail (ab110410)
ab110410 has not yet been referenced specifically in any publications.
Publishing research using ab110410? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"