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Products:Signal Transduction >> Metabolism >> Mitochondrial
MSCatalog No. MS301-250
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Read our guarantee »Complex III Immunocapture Kit
See all Complex III products (3) ...
Quantitative
Reacts with
Mouse, Rat, Cow, Human
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.
ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Store at +4°C.
| Components | 250 µg | 500 µg | 750 µg |
|---|---|---|---|
| monoclonal Complex III antibody irreversibly cross-linked to protein G-agarose beads | 1 x 250µg | 1 x 500µg | 1 x 750µg |
Immunoprecipitation - Complex III Immunocapture Kit (ab109800)
(enlarge)
Complex II (succinate-ubiquinone oxidoreductase), one of the mitochondrial respiratory chain complexes, transfers electrons from succinate generated during the citric acid cycle to Complex III (ubiquinolcytochrome c oxidoreductase), via a mobile electron shuttle, ubiquinone. Complex III transfers electrons to Complex IV (cytochrome c oxidase) via another mobile electron shuttle, cytochrome c.
Our Abpromise guarantee covers the use of ab109800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Immunoprecipitation - Complex III Immunocapture Kit (ab109800)

Complex III immunoprecipitation using antibody ab109862/MS101c crosslinked to protein G-agarose beads as product ab109800
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