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Read our guarantee »Overview
Product name
Complex III Immunocapture Kit
See all Complex III products (3) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.
ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Tested applications
Properties
Storage instructions
Store at +4°C.
| Components | 250 µg | 500 µg | 750 µg |
|---|---|---|---|
| monoclonal Complex III antibody irreversibly cross-linked to protein G-agarose beads | 1 x 250µg | 1 x 500µg | 1 x 750µg |
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Immunoprecipitation - Complex III Immunocapture Kit (ab109800)
(enlarge)
Relevance
Complex II (succinate-ubiquinone oxidoreductase), one of the mitochondrial respiratory chain complexes, transfers electrons from succinate generated during the citric acid cycle to Complex III (ubiquinolcytochrome c oxidoreductase), via a mobile electron shuttle, ubiquinone. Complex III transfers electrons to Complex IV (cytochrome c oxidase) via another mobile electron shuttle, cytochrome c.
Alternative names
- MS1037
- MTOX3
Database links
- Entrez Gene: 7384 Human
- Entrez Gene: 7385 Human
- Entrez Gene: 1537 Human
- Entrez Gene: 7386 Human
- Entrez Gene: 4519 Human
- Entrez Gene: 7381 Human
- Entrez Gene: 7388 Human
- Entrez Gene: 27089 Human
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Complex III Immunocapture Kit images:
Immunoprecipitation - Complex III Immunocapture Kit (ab109800)
Complex III immunoprecipitation using antibody ab109862/MS101c crosslinked to protein G-agarose beads as product ab109800
Protocols
References for Complex III Immunocapture Kit (ab109800)
This product has been referenced in:
- Korde AS et al. Primary role of mitochondrial Rieske iron-sulfur protein in hypoxic ROS production in pulmonary artery myocytes. Free Radic Biol Med 50:945-52 (2011).Read more (PubMed: 21238580) »
- Munusamy S et al. Alteration of renal respiratory Complex-III during experimental type-1 diabetes. BMC Endocr Disord 9:2 (2009).Read more (PubMed: 19166612) »
See all 3 publications for this product
Publishing research using ab109800? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"