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250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.
ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
|Components||250 µg||500 µg||750 µg|
|monoclonal Complex III antibody irreversibly cross-linked to protein G-agarose beads||1 x 250µg||1 x 500µg||1 x 750µg|
Our Abpromise guarantee covers the use of ab109800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
Complex III immunoprecipitation using antibody ab109862/MS301 crosslinked to protein G-agarose beads as product ab109800.