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MitoSciences (MS301)

Complex III Immunocapture Kit (ab109800)

Q&A (6)Specific References (3)

Overview

  • Product nameComplex III Immunocapture KitSee all Complex III kits ...
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.

    ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIP more details

Properties

  • RelevanceComplex II (succinate-ubiquinone oxidoreductase), one of the mitochondrial respiratory chain complexes, transfers electrons from succinate generated during the citric acid cycle to Complex III (ubiquinolcytochrome c oxidoreductase), via a mobile electron shuttle, ubiquinone. Complex III transfers electrons to Complex IV (cytochrome c oxidase) via another mobile electron shuttle, cytochrome c.
    • Alternative names
        coenzyme Q : cytochrome c — oxidoreductasecytochrome bc1 complex
  • Database links
    see all

    • Applications

    Our Abpromise guarantee covers the use of ab109800 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IP IP

    Complex III Immunocapture Kit images

    • Immunoprecipitation - Complex III Immunocapture Kit (ab109800)
      Immunoprecipitation - Complex III Immunocapture Kit (ab109800)
      Complex III immunoprecipitation using antibody ab109862/MS101c crosslinked to protein G-agarose beads as product ab109800

    Protocols

    References for Complex III Immunocapture Kit (ab109800)

    This product has been referenced in:
    • Korde AS  et al. Primary role of mitochondrial Rieske iron-sulfur protein in hypoxic ROS production in pulmonary artery myocytes. Free Radic Biol Med 50:945-52 (2011). Read more (PubMed: 21238580) »
    • Munusamy S  et al. Alteration of renal respiratory Complex-III during experimental type-1 diabetes. BMC Endocr Disord 9:2 (2009). Read more (PubMed: 19166612) »

    See all 3 Publications for this product

    Publishing research using ab109800 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab109800

    Displaying 1 - 6 of 6 results for Q&A

    10
    Votes: Highest First

    Dear Josephine

    Thank you for your reply.
    Our customer knows complex III have total 11 subunits. So he would like to know if rest of them couldn’t detectusing this kit.
    Please let me know.

    Thank you for contacting us.

    Yes, the antibody coated beads can pull down the whole complex but need to make sure your customer needs to use Lauryl-β-D-maltoside (http://www.abcam.com/10-Lauryl-Maltoside-Solution-ab109857.html) to solubiliz...

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    I have purchased the 250ug size of ab109800. What is the volume of the bead solution in this kit?

    The 250 ug size kit contains 25 uL of beads in a total volume of 400 uL. The buffer is 1X Hepes-buffered saline with 0.02% sodium azide preservative.

    I hope this is helpful. Please contact us again if you have any further questions.

    I have purchased the 250ug size of ab109800. What is the volume of the bead solution in this kit?

    We put 25 uL of beads in each unit.

    I hope this is helpful. Please contact me again if you have any further questions.

    What antibodies (and what targets) have you used in the WB following the IP?

    Thank you very much for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

    After the IP, you can use Ab110252 (UQCRC1), Ab14745(UQCRC2), Ab110255 (UQRCRQ) or Ab14746(UQCRFS1) as follow up an...

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     I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

    The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Western ...

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     I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

    Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue requi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"