Overview

  • Product nameComplex III Immunocapture Kit
    See all Complex III kits
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.

    ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIPmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab109800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.

Complex III Immunocapture Kit images

  • Complex III immunoprecipitation using antibody ab109862/MS301 crosslinked to protein G-agarose beads as product ab109800.

Protocols

References for Complex III Immunocapture Kit (ab109800)

This product has been referenced in:
  • Korde AS  et al. Primary role of mitochondrial Rieske iron-sulfur protein in hypoxic ROS production in pulmonary artery myocytes. Free Radic Biol Med 50:945-52 (2011). Read more (PubMed: 21238580) »
  • Munusamy S  et al. Alteration of renal respiratory Complex-III during experimental type-1 diabetes. BMC Endocr Disord 9:2 (2009). Read more (PubMed: 19166612) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

Yes, the antibody coated beads can pull down the whole complex but need to make sure your customer needs to use Lauryl-β-D-maltoside (http://www.abcam.com/10-Lauryl-Maltoside-Solution-ab109857.html) to solubi...

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The 250 ug size kit contains 25 uL of beads in a total volume of 400 uL. The buffer is 1X Hepes-buffered saline with 0.02% sodium azide preservative.

I hope this is helpful. Please contact us again if you have any further questions.

We put 25 uL of beads in each unit.

I hope this is helpful. Please contact me again if you have any further questions.

Thank you very much for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

After the IP, you can use Ab110252 (UQCRC1), Ab14745(UQCRC2), Ab110255 (UQRCRQ) or Ab14746(UQCRFS1) as follow up...

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The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Weste...

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Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"