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Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)

MSCatalog No. MS301c

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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Overview

Product name

Anti-Complex III Immunocapture antibody [11A51H12]

Description

Mouse monoclonal [11A51H12] to Complex III Immunocapture

Specificity

100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria.

Tested applications

IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Bovine Heart Complex III

Positive control

Bovine heart and Human mitochondria

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purification notes

ab10862 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

11A51H12

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial

  • Immunoprecipitation - Complex III Immunocapture antibody [11A51H12] (ab109862)Immunoprecipitation - Complex III Immunocapture antibody [11A51H12] (ab109862) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab109862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Complex III, or ubiquinol cytochrome c oxidoreductase, is a complex of 11 subunits and catalyses electron transfer from reduced ubiquinone or coenzyme Q 10 to cytochrome c. The complex contains 11 different subunits, one of which, cytochrome b, is encoded on mitochondrial DNA, while the remainder subunits are nuclear-encoded. Genetic complex III deficiencies occur but are rare.

Anti-Complex III Immunocapture antibody [11A51H12] images:

  Immunoprecipitation - Complex III Immunocapture antibody [11A51H12] (ab109862)

Immunoprecipitation - Complex III Immunocapture antibody [11A51H12] (ab109862)

Staining of Complex III Lane 1: 0.1mg of Bovine mitochondria Lane 2: 0.05mg Bovine mitochondria Lane 3: 0.05mg Human mitochondria Immunoprecipitated using ab109862 at 10 µg/mg lysate.

References for Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)

This product has been referenced in:

  • Schilling B  et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006).Read more (PubMed: 16120479) »

See 1 publication for this product

Publishing research using ab109862? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"