Complex IV Human Enzyme Activity Microplate Assay Kit (ab109909)
- Product nameComplex IV Human Enzyme Activity Microplate Assay KitSee all Complex IV kits ...
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue
- Assay typeEnzyme activity
- Species reactivityReacts with: Cow, Human
Does not react withMouse, Rat
- Product overview
The Complex IV Human Enzyme Activity Microplate Assay Kit is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Store Tube 1, detergent, and microplate at 4°C – DO NOT FREEZE. Store Reagent C at -20°C or at -80°C for long-term storage.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 96 tests 96-well microplate (12 strips) 1 unit Buffer 1 x 10ml Detergent 1 x 1ml Reagent C (Cytochrome c) 1 x 1ml
- Research Areas
- COXCytochrome c oxidaseCytochrome oxidase
- Entrez Gene: 4512 Human
- Entrez Gene: 4513 Human
- Entrez Gene: 4514 Human
- Entrez Gene: 1345 Human
- Entrez Gene: 1351 Human
- Entrez Gene: 1329 Human
- Entrez Gene: 1349 Human
- Entrez Gene: 1337 Human
- Entrez Gene: 1327 Human
- Entrez Gene: 1340 Human
- Entrez Gene: 1350 Human
- Entrez Gene: 9377 Human
- Entrez Gene: 1346 Human
- SwissProt: P00395 Human
- SwissProt: P00403 Human
- SwissProt: P00414 Human
- SwissProt: P13073 Human
- SwissProt: P12074 Human
- SwissProt: P15954 Human
- SwissProt: P24311 Human
- SwissProt: P10176 Human
- SwissProt: P14854 Human
- SwissProt: P09669 Human
- SwissProt: P24310 Human
- SwissProt: P20674 Human
- SwissProt: P10606 Human
Our Abpromise guarantee covers the use of ab109909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Complex IV Human Enzyme Activity Microplate Assay Kit images
Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.
Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, substrate is added, and enzyme activity is analyzed by measuring the change in absorbance of either the substrate or the product of the reaction (depending upon which enzyme is being analyzed). By analyzing the enzyme's activity in an isolated context, outside of the cell and free from any other variables, an accurate measurement of the enzyme's functional state can be understood.
Figure 1. To determine the activity in the sample, calculate the slope by using microplate software or by manual calculations using one of the two methods shown. Compare the sample rate with the rate of the control (normal) sample and with the rate of the null (background) to get the relative Complex IV activity. (A)The rate is determined by calculating the gradient of the initial slope over the linear region. (B) The rate is determined by calculating the slope between two points within the linear region.
References for Complex IV Human Enzyme Activity Microplate Assay Kit (ab109909)
This product has been referenced in:
- Wu C et al. Cytochrome c oxidase III as a mechanism for apoptosis in heart failure following myocardial infarction. Am J Physiol Cell Physiol 297:C928-34 (2009). Macaque Monkey . Read more (PubMed: 19625613) »
- Tsutsumi T et al. Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperon, prohibitin, in cells expressing hepatitis C virus core protein. Hepatology 50:378-86 (2009). Read more (PubMed: 19591124) »