Complex IV Human Protein Quantity Dipstick Assay Kit (ab109877)
- Product nameComplex IV Human Protein Quantity Dipstick Assay KitSee all Complex IV kits ...
- Sample typeCell culture extracts, Tissue
- Assay typeQuantitative
- Species reactivityReacts with: Cow, Human
- Product overview
ab109877(MS431) contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled cytochrome c oxidase enzyme complex from human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
Based on the immunologic sandwich assay, this kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the intact COX enzyme complex. One antibody is immobilized on nitrocellulose membrane in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated for a visual signal. When assembled COX is present in the sample, a red line appears on the dipstick at the site of the COX antibody line. The signal intensity is directly related to the level of COX enzyme complex in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 90 tests 30 tests Buffer B (10X Blocking solution) 1 x 1.2ml 1 x 0.4ml Buffer C (Wash buffer) 1 x 3ml 1 x 1ml Dipsticks 1 x 90 units 1 x 30 units Extraction Buffer 1 x 45ml 1 x 15ml Gold-conjugated antibody (dried in microplate wells) 1 x 90 tests 1 x 30 tests
- Research Areas
- COXCytochrome c oxidaseCytochrome oxidase
- Entrez Gene: 4512 Human
- Entrez Gene: 4513 Human
- Entrez Gene: 4514 Human
- Entrez Gene: 1345 Human
- Entrez Gene: 1351 Human
- Entrez Gene: 1329 Human
- Entrez Gene: 1349 Human
- Entrez Gene: 1337 Human
- Entrez Gene: 1327 Human
- Entrez Gene: 1340 Human
- Entrez Gene: 1350 Human
- Entrez Gene: 9377 Human
- Entrez Gene: 1346 Human
- SwissProt: P00395 Human
- SwissProt: P00403 Human
- SwissProt: P00414 Human
- SwissProt: P13073 Human
- SwissProt: P12074 Human
- SwissProt: P15954 Human
- SwissProt: P24311 Human
- SwissProt: P10176 Human
- SwissProt: P14854 Human
- SwissProt: P09669 Human
- SwissProt: P24310 Human
- SwissProt: P20674 Human
- SwissProt: P10606 Human
Our Abpromise guarantee covers the use of ab109877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Complex IV Human Protein Quantity Dipstick Assay Kit images
An example using ab109877 to quantify Complex IV levels using various concentration of human fibroblast extract. Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 10 µg of fibroblast extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.
An example using ab109877 to quantify Complex IV levels using various concentration of human fibroblast extract. Based on the above standard curve values, 5 µg of protein extract were loaded to each dipstick for the four unknown samples (1-4). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of Complex I in samples 1-5 was determined. Based on the above analysis, the unknown samples had between 4% and 64% of Complex IV levels compared to the control.
Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.
References for Complex IV Human Protein Quantity Dipstick Assay Kit (ab109877)
This product has been referenced in:
- Nadanaciva S et al. Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis. J Immunol Methods 343:1-12 (2009). Read more (PubMed: 19152798) »
- Vercauteren K et al. Short hairpin RNA-mediated silencing of PRC (PGC-1-related coactivator) results in a severe respiratory chain deficiency associated with the proliferation of aberrant mitochondria. J Biol Chem 284:2307-19 (2009). Read more (PubMed: 19036724) »