MitoSciences (MS443)

Complex IV Human Specific Activity Microplate Assay Kit (ab109910)

Overview

  • Product nameComplex IV Human Specific Activity Microplate Assay KitSee all Complex IV kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeEnzyme activity
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    This rapid multiplexing microplate kit is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. Subsequently, in these same wells the quantity of the enzyme can then be determined by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody; this phosphatase changes a substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the multiplexing assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

  • Tested applicationsFunctional Studiesmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab109910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Complex IV Human Specific Activity Microplate Assay Kit images

  • Functional study using Complex IV Human Specific Activity Microplate Assay Kit (ab109910).
  • Functional study using Complex IV Human Specific Activity Microplate Assay Kit (ab109910).
  • Principle of Sandwich ELISA
  • Principle of Complex IV Human Specific Activity Microplate Assay Kit (ab109910)

Protocols

References for Complex IV Human Specific Activity Microplate Assay Kit (ab109910)

This product has been referenced in:
  • Pacheu-Grau D  et al. Mitochondrial antibiograms in personalized medicine. Hum Mol Genet 22:1132-9 (2013). Human . Read more (PubMed: 23223015) »
  • Lorente L  et al. Survival and mitochondrial function in septic patients according to mitochondrial DNA haplogroup. Crit Care 16:R10 (2012). Human . Read more (PubMed: 22251664) »

See all 5 Publications for this product

Product Wall

Yes, we use 10% detergent in ab109910 which is available as ab109857 so the final is 1%. This detergent is pretty mild and yields native functional enzymes.

Also, a final of 1.5% could be used (i.e. extraction buffer) without significantly c...

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The slope to measure is the fast decreasing slope. The cytochrome c oxidase activity assay measures the capacity of the immunocapture enzyme for oxidizing reduced cytochrome c. Reduced cytochrome c is measured at 550nm. A reduction in the reading means...

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We have not tested the enzyme activity portion of the assay (ab109910) after overnight incubation at 4C. My suggestion is to not do it and stick to the conditions set out in the protocol = 3 hours incubation of sample at RT – then perform the act...

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Thank you for contacting us.

You may be interested in our cytochrome c protein quantity microplate assay kit, ab110172.

http://www.abcam.com/ps/products/110/ab110172/documents/ab110172%20Cytochrome%20c%20Protein%20Quantity%20micro...

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Answer 1. Mitochondrial isolation can be performed according to kits ab110169 or ab110171.

2. Answer 2. Solution 1 does not contain succinate; instead the cytochrome c has been reduced with DTT and then purified.

Answer 3. BCA can b...

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Thank you for contacting us.

Please find attached the sample preparation guide recommended by our Mitoscience colleagues.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or i...

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Thank you for calling Abcam. Please find attached the MSDS for ab109910 and ab110671. If there is anything else I can do, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"