Complex IV Human Specific Activity Microplate Assay Kit (ab109910)

Overview

• Product nameComplex IV Human Specific Activity Microplate Assay KitSee all Complex IV kits ...
• Tests
  1 x 96 well plate
• Sample type
  Cell culture extracts, Tissue
• Assay typeEnzyme activity
• Species reactivity
  Reacts with: Cow, Human
  
• Product overview

  This rapid multiplexing microplate kit is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. Subsequently, in these same wells the quantity of the enzyme can then be determined by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody; this phosphatase changes a substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the multiplexing assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	Identifier	96 tests
  20X Buffer		1 x 15ml
  96-well microplate (12 strips)		1 unit
  AP Development Reagent		1 x 400µl
  AP label 2500X	Tube B	1 x 12µl
  Detector antibody	Tube A	1 x 1ml
  Detergent		1 x 1ml
  Development buffer	Tube 3	1 x 10ml
  Reagent C (Cytochrome c)		1 x 1ml
  Wash Buffer	Tube 2	1 x 2ml

• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Cytochromes

  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Other Metabolism Assay

  • Signal Transduction
  • Metabolism
  • Mitochondrial

Applications

Complex IV Human Specific Activity Microplate Assay Kit images

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  Functional Studies - Complex IV Human Specific Activity Microplate Assay Kit (ab109910)
  Functional study using Complex IV Human Specific Activity Microplate Assay Kit (ab109910).
• 

  Functional Studies - Complex IV Human Specific Activity Microplate Assay Kit (ab109910)
  Functional study using Complex IV Human Specific Activity Microplate Assay Kit (ab109910).
• 

  Sandwich ELISA - Complex IV Human Specific Activity Microplate Assay Kit (ab109910)
  Principle of Sandwich ELISA
• 

  Functional Studies - Complex IV Human Specific Activity Microplate Assay Kit (ab109910)
  Principle of Complex IV Human Specific Activity Microplate Assay Kit (ab109910)

Protocols

Protocol Booklet