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Complex IV Immunocapture Kit (ab109801)

MSCatalog No. MS401-250

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Overview

Product name

Complex IV Immunocapture Kit
See all Complex IV products (10) ...

Assay type

Quantitative

Cross reactivity

Reacts with

Cow, Human

Product overview

250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.

ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.

Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

Tested applications

IPmore details

Properties

Storage instructions

Store at +4°C.

Components 250 µg 500 µg 750 µg
monoclonal Complex IV antibody irreversibly crosslinked to protein G-agarose beads1 x 250µg1 x 500µg1 x 750µg

Research areas

Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Mitochondrial

  • Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)Immunoprecipitation - Complex IV Immunocapture Kit (ab109801) image (enlarge)

Alternative names

  • Cytochrome c oxidase
  • MS1038
  • MS441
  • MTOX4

Database links

see all

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Complex IV Immunocapture Kit images:

  Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)

Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)

Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801

Protocols

  • Protocol Booklet

    • References for Complex IV Immunocapture Kit (ab109801)

    This product has been referenced in:

    • van den Bosch BJ  et al. Defective NDUFA9 as a novel cause of neonatally fatal complex I disease. J Med Genet 49:10-5 (2012).Read more (PubMed: 22114105) »
    • Tucker EJ  et al. Mutations in MTFMT Underlie a Human Disorder of Formylation Causing Impaired Mitochondrial Translation. Cell Metab 14:428-34 (2011).Read more (PubMed: 21907147) »

    See all 6 publications for this product

    Publishing research using ab109801? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"