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MitoSciences (MS401)

Complex IV Immunocapture Kit (ab109801)

Q&A (3)Specific References (6)

Overview

  • Product nameComplex IV Immunocapture KitSee all Complex IV kits ...
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.

    ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIP more details

Properties

  • Alternative names
      COXCytochrome c oxidaseCytochrome oxidase
  • Database links
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    • Applications

    Our Abpromise guarantee covers the use of ab109801 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IP IP

    Complex IV Immunocapture Kit images

    • Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)
      Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)
      Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801

    Protocols

    References for Complex IV Immunocapture Kit (ab109801)

    This product has been referenced in:
    • van den Bosch BJ  et al. Defective NDUFA9 as a novel cause of neonatally fatal complex I disease. J Med Genet 49:10-5 (2012). Read more (PubMed: 22114105) »
    • Tucker EJ  et al. Mutations in MTFMT Underlie a Human Disorder of Formylation Causing Impaired Mitochondrial Translation. Cell Metab 14:428-34 (2011). Read more (PubMed: 21907147) »

    See all 6 Publications for this product

    Publishing research using ab109801 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 3 of 3 results for Abreviews and Q&A

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    Customer kindly contacted us to determine if this product might work with zebrafish or goldfish samples.

    Thank you for contacting us.

    I have been in contact with the lab regarding species reactivity of this product as you have requested. They have tested the paired antibody in zebrafish and this will definitely not work on Zebrafish or goldfish....

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     I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

    The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Western ...

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     I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

    Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue requi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"