Overview

  • Product nameComplex IV Immunocapture Kit
    See all Complex IV kits
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.

    ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIPmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab109801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.

Complex IV Immunocapture Kit images

  • Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801

Protocols

References for Complex IV Immunocapture Kit (ab109801)

This product has been referenced in:
  • van den Bosch BJ  et al. Defective NDUFA9 as a novel cause of neonatally fatal complex I disease. J Med Genet 49:10-5 (2012). Read more (PubMed: 22114105) »
  • Tucker EJ  et al. Mutations in MTFMT Underlie a Human Disorder of Formylation Causing Impaired Mitochondrial Translation. Cell Metab 14:428-34 (2011). Read more (PubMed: 21907147) »

See all 6 Publications for this product

Product Wall

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I have been in contact with the lab regarding species reactivity of this product as you have requested. They have tested the paired antibody in zebrafish and this will definitely not work on Zebrafish or goldfi...

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The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Weste...

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Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"