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Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)

MSCatalog No. MS401c

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Overview

Product name

Anti-Complex IV Immunocapture antibody [31E91B82G9]

Description

Mouse monoclonal [31E91B82G9] to Complex IV Immunocapture

Specificity

100 µg ab109863 can capture at least 10 µg Complex IV (COX) from 1 mg solubilized Bovine heart mitochondria.

Tested applications

IPmore details

Cross reactivity

Reacts with

Cow, Human

Immunogen

Bovine Complex IV

Positive control

Bovine heart and Human mitochondria

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purification notes

ab109863 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

31E91B82G9

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial

  • Immunoprecipitation - Complex IV Immunocapture antibody [31E91B82G9] (ab109863)Immunoprecipitation - Complex IV Immunocapture antibody [31E91B82G9] (ab109863) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for ,IHC-P or WB.

Target

Relevance

Complex IV, also called cytochrome c oxidoreductase or cytochrome c oxidase, is a complex of 13 different subunits, three of which (I, II and III) are encoded on mitochondrial DNA and the remainder in the nuclear DNA. The complex contains two heme groups (a and a3) and two copper atoms as prosthetic groups. Genetic alterations of this enzyme complex are a common cause of OXPHOS diseases and the enzyme is altered in patients with Alzheimer’s disease. Also, there are reports of reduced amounts of this complex in hypoxic cancer cells.

Cellular localization

Mitochondrial

Alternative names

  • MS401c antibody

Anti-Complex IV Immunocapture antibody [31E91B82G9] images:

  Immunoprecipitation - Complex IV Immunocapture antibody [31E91B82G9] (ab109863)

Immunoprecipitation - Complex IV Immunocapture antibody [31E91B82G9] (ab109863)

Staining of Complex IV Lane 1: 0.1 mg Human mitochondrial lysate Lane 2: 0.1 mg Bovine mitochondrial lysate Lane 3: 0.025 mg Human mitochondrial lysate Lane 4: 0.025 mg Bovine mitochondrial lysate Immunoprecipitated using ab109863 at 100 µg/mg lysate

References for Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)

This product has been referenced in:

  • Escobar-Alvarez S  et al. Inhibition of human peptide deformylase disrupts mitochondrial function. Mol Cell Biol 30:5099-109 (2010).Read more (PubMed: 20805355) »
  • Nadanaciva S  et al. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays. Toxicol In Vitro 21:902-11 (2007).Read more (PubMed: 17346924) »

See all 2 publications for this product

Publishing research using ab109863? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"