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MitoSciences (MS402)

Complex IV Rodent Immunocapture Kit (ab109860)

Q&A (4)Specific References (1)

Overview

  • Product nameComplex IV Rodent Immunocapture KitSee all Complex IV kits ...
  • Species reactivity
    Reacts with: Mouse, Rat
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.

    ab109860 allows for isolation of the cytochrome c oxidase complex from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109860 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIP more details

Properties

  • Alternative names
      COXCytochrome c oxidaseCytochrome oxidase

Applications

Our Abpromise guarantee covers the use of ab109860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP

Protocols

References for Complex IV Rodent Immunocapture Kit (ab109860)

This product has been referenced in:
  • Strub GM  et al. Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration. FASEB J 25:600-12 (2011). Read more (PubMed: 20959514) »

See 1 Publication for this product

Publishing research using ab109860 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 4 of 4 results for Abreviews and Q&A

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Hello.
I have purchased the product: Complex IV Rodent Immunocapture Kit. After several experiemnts with their
respective controls we have conclude that is not working. May be during the delivery proccess the Kit
have been spoiled or damage...

Read More

I am very sorry but, based on the lot number you provided, it appears you receivedthe IP reagent for ab109801, which only works with HUMAN and COW samples. Youunfortunately did not receive the IP reagent forab109860, a complex IV immunocapture kit suitabl...

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Hello. I have purchased the product: Complex IV Rodent Immunocapture Kit. After several experiemnts with their respective controls we have conclude that is not working. May be during the delivery proccess the Kit have been spoiled or damaged as Complex I ...

Read More

I am very sorry to hear there is a problem here. If you could, a few additional details would be helpful to help us determine what the problems may be: 1. Canyou addmolecular weight markers and show the full gel so we can see the top of the blot. 2.What ...

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Hello. I attach a file from IP experiments. You`ll see how while using Complex I Immunocapture kit i succeed in Immunocapturing CI (WB against NDUFA9) but i also Immunocapture complex IV, (WB against COX4), but with Complex IV Immunocapture ki,t i Immunoc...

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Thank you for sending along your IP/western blot data. It does appear the IP of  complex IV is not working. If you have purchased this kit within the last 6 months or so I would be happy to send you a new kit. So I can process your request, please include...

Read More

Hello. I have purchased the product: Complex IV Rodent Immunocapture Kit. After several experiemnts with their respective controls we have conclude that is not working. May be during the delivery proccess the Kit have been spoiled or damaged as Complex I ...

Read More

Thank you for your enquiry. I'm sorry to hear the Complex IV Immunocapture kit is giving you trouble. I think it would be helpful if you could give a few more details describing how the kit is not working. Including an attached figure would be quite usefu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"