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250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
ab109860 allows for isolation of the cytochrome c oxidase complex from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109860 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
|Components||250 µg||500 µg|
|monoclonal antibody irreversibly crosslinked to protein G-agarose beads||1 x 250µg||1 x 500µg|
Our Abpromise guarantee covers the use of ab109860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|