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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Overview
Product name
Anti-Constitutive androstane receptor antibody
See all Constitutive androstane receptor products (6) ...
Description
Rabbit polyclonal to Constitutive androstane receptor
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chimpanzee
Immunogen
Synthetic peptide: MASREDELRN CVVCGDQATG YHFNALTCEG CKGFFRRTVS KSIGPTCPFA, corresponding to a region within internal sequence amino acids 1-50 of Human Constitutive androstane receptor
MASREDELRN CVVCGDQATG YHFNALTCEG CKGFFRRTVS KSIGPTCPFA
Positive control
Human liver tissue. Fetal liver cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
IHC-P: Use a concentration of 4.0 - 8.0 µg/ml.
Target
Relevance
Constitutive androstane receptor (CAR) is a member of orphan receptor. CAR is mainly expressed in liver and lower levels of expression are observed in heart, muscles, kidney and lung. CYP3A and CYP2B are regulated by CAR, which suggest xenobiotics can activate multiple CYP genes through this receptor. Mouse homolog is induced by phenobarbital and CAR-null mouse demonstrate resistance to acetaminophen toxicity. CAR affects metabolism of drugs and other xenobiotics.
Cellular localization
Nucleus (By similarity)
Alternative names
- CAR antibody
- CAR BETA antibody
- CAR1 antibody
see all
Database links
- Entrez Gene: 9970 Human
- Entrez Gene: 12355 Mouse
- Entrez Gene: 65035 Rat
- Omim: 603881 Human
- SwissProt: Q14994 Human
- SwissProt: O35627 Mouse
- SwissProt: Q402B9 Rat
- SwissProt: Q402C0 Rat
see all
Anti-Constitutive androstane receptor antibody images:
Western blot - Constitutive androstane receptor antibody (ab62590)
Anti-Constitutive androstane receptor antibody (ab62590) at 1.25 µg/ml + fetal liver cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 40 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Antibody diluted in 5% skim milk / PBS buffer.
Gel concentration: 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Constitutive androstane receptor antibody (ab62590)
ab62590, at 4.0µg/ml, staining Constitutive androstane receptor in human liver by immunohistochemistry using paraffin-embedded tissue. The arrows indicate positively stained hepatocytes.
References for Anti-Constitutive androstane receptor antibody (ab62590)
ab62590 has not yet been referenced specifically in any publications.
Publishing research using ab62590? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"