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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Overview
Product name
Anti-Constitutive androstane receptor antibody
See all Constitutive androstane receptor products (6) ...
Description
Rabbit polyclonal to Constitutive androstane receptor
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from an internal sequence of human Constitutive androstane receptor
Positive control
Jurkat cell extracts treated with serum (20%, 15mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - Constitutive androstane receptor antibody (ab72709)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Relevance
Constitutive androstane receptor (CAR) is a member of orphan receptor. CAR is mainly expressed in liver and lower levels of expression are observed in heart, muscles, kidney and lung. CYP3A and CYP2B are regulated by CAR, which suggest xenobiotics can activate multiple CYP genes through this receptor. Mouse homolog is induced by phenobarbital and CAR-null mouse demonstrate resistance to acetaminophen toxicity. CAR affects metabolism of drugs and other xenobiotics.
Cellular localization
Nucleus (By similarity)
Alternative names
- CAR antibody
- CAR BETA antibody
- CAR1 antibody
see all
Database links
- Entrez Gene: 9970 Human
- Omim: 603881 Human
- SwissProt: Q14994 Human
Anti-Constitutive androstane receptor antibody images:
Western blot - Constitutive androstane receptor antibody (ab72709)
All lanes : Anti-Constitutive androstane receptor antibody (ab72709) at 1/500 dilution
Lane 1 : Jurkat cell extracts treated
with serum (20%, 15mins)
Lane 2 : Jurkat cell extracts treated
with serum (20%, 15mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 40 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
References for Anti-Constitutive androstane receptor antibody (ab72709)
ab72709 has not yet been referenced specifically in any publications.
Publishing research using ab72709? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"