Recombinant Anti-CPOX antibody [EPR11926(B)] (ab170945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11926(B)] to CPOX
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CPOX antibody [EPR11926(B)]
See all CPOX primary antibodies -
Description
Rabbit monoclonal [EPR11926(B)] to CPOX -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CPOX aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P36551 -
Positive control
- HepG2, K562, LnCaP, and Jurkat cell lysates; HepG2 and K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11926(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III. -
Pathway
Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. -
Involvement in disease
Defects in CPOX are the cause of hereditary coproporphyria (HCP) [MIM:121300]. HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity. -
Sequence similarities
Belongs to the aerobic coproporphyrinogen-III oxidase family. -
Cellular localization
Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 1371 Human
- Omim: 612732 Human
- SwissProt: P36551 Human
- Unigene: 476982 Human
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Alternative names
- Coprogen oxidase antibody
- COPROPORPHYRIA antibody
- Coproporphyrinogen III oxidase antibody
see all
Images
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All lanes : Anti-CPOX antibody [EPR11926(B)] (ab170945) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
Immunofluorescence analysis of HepG2 cells labeling CPOX with ab170945 at 1/100 (red). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of K562 cells labeling CPOX with ab170945 at 1/10 dilution (red) and rabbit IgG (negative control) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170945 has not yet been referenced specifically in any publications.