Anti-Coronin 1a/TACO antibody - C-terminal (ab228635)
Key features and details
- Rabbit polyclonal to Coronin 1a/TACO - C-terminal
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Coronin 1a/TACO antibody - C-terminal
See all Coronin 1a/TACO primary antibodies -
Description
Rabbit polyclonal to Coronin 1a/TACO - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide within Human Coronin 1a/TACO (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P31146 -
Positive control
- WB: U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: HepG2 cells. IHC-P: Human gastric cancer; mouse and rat brain tissue. Mouse duodenum and rat intestine tissue. IHC-Fr: Mouse cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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IHC-P |
1/100 - 1/1000.
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IHC-Fr |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
IHC-P
1/100 - 1/1000. |
IHC-Fr
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. -
Tissue specificity
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification. - Information by UniProt
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Database links
- Entrez Gene: 11151 Human
- Entrez Gene: 12721 Mouse
- Entrez Gene: 155151 Rat
- Omim: 605000 Human
- SwissProt: P31146 Human
- SwissProt: O89053 Mouse
- SwissProt: Q91ZN1 Rat
- Unigene: 415067 Human
see all -
Alternative names
- Actin binding protein antibody
- CLABP antibody
- Clipin A antibody
see all
Images
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All lanes : Anti-Coronin 1a/TACO antibody - C-terminal (ab228635) at 1/1000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (Human brain neuroblastoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE gel.
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Frozen-sectioned adult mouse cerebellum stained for Coronin 1A/TACO Protein (green) using ab228635 at 1/500 in immunohistochemical analysis.
NF-H (Neurofilament Heavy) cells stained for NF-H (red) using NF-H antibody at 1/500 dilution.
Counterstain: Fluoroshield with DAPI (blue).
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Paraffin-embedded rat intestine tissue stained for Coronin 1A/TACO protein using ab228635 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse duodenum tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human gastric cancer tissue stained for Coronin 1A/TACO using ab228635 at 1/100 dilution in immunohistochemical analysis.
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Methanol-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Coronin 1A/TACO (green) using ab228635 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228635 has not yet been referenced specifically in any publications.