Overview

  • Product nameCorticosterone ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.6%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.28 ng/ml
  • Range
    2 ng/ml - 22 ng/ml
  • Recovery

    = 101 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
  • Product overview

    Abcam’s Corticosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.

    A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCorticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.

Applications

Our Abpromise guarantee covers the use of ab108821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Corticosterone ELISA kit images

  • Competitive ELISA with ab108821.
  • Corticosterone measured in biological fluids showing quantity (ng) per mL of tested sample

Protocols

References for Corticosterone ELISA kit (ab108821)

This product has been referenced in:
  • Medwid S  et al. Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring. Environ Toxicol Pharmacol 43:203-8 (2016). Mouse . Read more (PubMed: 27017381) »
  • Lee B  et al. Effects of systemic administration of ibuprofen on stress response in a rat model of post-traumatic stress disorder. Korean J Physiol Pharmacol 20:357-66 (2016). Read more (PubMed: 27382352) »

See all 13 Publications for this product

Product Wall

We are happy to help! :-)
I wish you success with your experiments and a good weekend.

Thank you for your email.
I am happy to send you the results of your experiments today. They were put in our program by one of our Lab members that is responsible for our ELISA testing and optimizing etc. As discussed on the phone this is a one-of...

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Thank you for contacting us.
The kit ab108821 utilizes the corticosterone specific antibodies. This kit cannot be used to detect the CBG (transcortin) corticosteroid-binding globulin in samples.
I hope this information is helpful to you. Plea...

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Thank you for contacting us. Unfortunately, this kit has not been tested for cross-reactivity with 11-DHC, so we do not have any information about whether or not it would cross-react. I am sorry I could not be more helpful, please let me know if you ha...

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Comme indiqué sur les fiches techniques respectives, ces 2 kits fonctionnent en effetavec des échantillons de sérum de rat.

http://www.abcam.com/ab108821

http://www.abcam.com/ab100578

Thank you for contacting us.
I think the results that the customer got is perfectly normal. ab108821 is a "competitive" ELISA. Step 2 of the flowchart on page 5 of the protocol, standard or sample is added together with a "biotinylated corticoster...

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Thank you for contacting us.

I have checked with my colleague, and could offer you either 20% off a primary antibody OR 20% off a protein/peptide product - for trying the kit in mouse brain.

Please let me know which one of these of...

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I am very pleased to hear you would like to accept our offer and test ab108821 in brain. This code will give you the price of the ELISA kit off your next purchasebefore the expiration date. To redeem this offer, please submit an Abreview for brain and ...

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Thank you for sending the image. We are looking into the labels for this to make sure it doesn't happen again. I can confirm that the brown glass vial does in fact contain the Biotinylated Corticosterone. This kit should be fine to use, and will be ...

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Thank you for contacting us. After checking with our stock team, they have informed me that the vials arrive already aliquotted by the lab and are then labelled before shipping. For this reason, it is more likely that the vials were not label...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"