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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Corticotropin Releasing Factor Receptor 2 antibody (ab12964)
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Immunocytochemistry/ Immunofluorescence - Corticotropin Releasing Factor Receptor 2 antibody (ab12964)
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Product Name
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Corticotropin Releasing Factor Receptor 2 antibody
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See all Corticotropin Releasing Factor Receptor 2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Corticotropin Releasing Factor Receptor 2
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Immunogen
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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive). (Peptide available as ab80010.)
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Reacts with
(species key)
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Hu, Rat, Shp
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Specificity
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This antibody recognizes both the alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2
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Epitope
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N-terminus (extracellular)
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Tested applications
(see key)
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ICC/IF, IHC (PFA fixed), IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunohistochemistry (PFA fixed): 1/4000 (from PMID 17586086).
ICC/IF: Suggested working dilution of 1/4000 (see PMID: 16337313 and images below).
IHC-P: 1/100 - 1/200.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Multi-pass membrane protein.
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Research areas
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Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Relevance
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Corticotropin Releasing Factor Receptor 2 (CRHR2) expression has been reported in various regions of the brain, as well as in placenta, umbilical vein, heart, epididymis, gastrointestinal tract, adrenal, and skeletal muscle. It shows high-affinity CRF binding and also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. CRCH2 alpha is the dominant isoform and is expressed widely. CRCH2 beta is expressed in the hippocampus, septum, amygdala, heart, and skeletal muscle. CRHR2 gamma is brain-specific. ESTs have been isolated from brain libraries.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.7 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Corticotropin Releasing Factor Receptor 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Corticotropin Releasing Factor Receptor 2 antibody images, references, products related to ab12964 and other tools.
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Corticotropin Releasing Factor Receptor 2 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Corticotropin Releasing Factor Receptor 2 antibody (ab12964)
Ab12964 positively staining formalin fixed paraffin embedded pituitary.
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Immunocytochemistry/ Immunofluorescence - Corticotropin Releasing Factor Receptor 2 antibody (ab12964)
ab12964 (CRF Rreceptor 2 antibody) immunofluorescence staining detecting hypothalamic paraventricular CRF Receptor 2 - containing neurones in rat brain (ab12964 at a dilution of 1/4000; FITC-labeled secondary antibody 1/100). This image was kindly supplied as part of the review submitted by Dr Christophe Porsher.
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References for Corticotropin Releasing Factor Receptor 2 antibody (ab12964)
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This product has been used in: (two most recent references)
Markovic D et al. Intracellular mechanisms regulating corticotropin-releasing hormone receptor-2beta endocytosis and interaction with extracellularly regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling cascades. Mol Endocrinol 22:689-706 (2008). WB; Human. PubMed: 18048641
Wu SV et al. Identification and characterization of multiple corticotropin-releasing factor type 2 receptor isoforms in the rat esophagus. Endocrinology 148:1675-87 (2007). PubMed: 17218420
See all 7 publication references for this product.
If you publish research using ab12964 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Corticotropin Releasing Factor Receptor 2
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Corticotropin Releasing Factor Receptor 2 antibody - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This product is sold under license from MBL International Corporation.
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