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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab75168)
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Related products
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab75168? Please let us know so that we can cite the reference in this datasheet
ab75168 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Corticotropin Releasing Factor Receptor 2 antibody (ab75168)
All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab75168) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from LOVO
cells
Lane 3 : extracts from 3T3 cells
Lane 4 : extracts from RAW264.7 cells
Lane 5 : extracts from 3T3 cells with immunising peptide at 10ug
Lysates/proteins at 30 µg per lane.
Predicted band size : 48 kDa
Observed band size : 48 kDa
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Corticotropin Releasing Factor Receptor 2 antibody(ab75168)
IHC image of ab75168 staining in human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab75168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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